三氧化二砷对人脑SHG-44胶质瘤细胞作用的体外实验研究  

Experimental study of inhibitory effect of arsenic trioxide on human SHG-44 glioma cells in Vitro

作　　者：郭云宝[1] 韩斌[1] 杨洪发[1] 

机构地区：[1]吉林大学白求恩第一医院神经外科,吉林长春130021

出　　处：《中风与神经疾病杂志》2013年第4期365-368,共4页Journal of Apoplexy and Nervous Diseases

摘　　要：目的探讨三氧化二砷(As2O3)对人脑SHG-44胶质瘤细胞株的增殖抑制及诱导凋亡机制。方法将不同浓度的As2O3与体外培养的SHG-44胶质瘤细胞相互作用后,采用MTT比色法检测As2O3对胶质瘤细胞的增殖抑制;倒置显微镜、荧光显微镜下观察吉姆萨及Hochest33258染色后细胞凋亡的形态学改变;流式细胞仪检测细胞凋亡及对SHG-44胶质瘤细胞周期影响。结果 MTT比色法检测到As2O3对SHG-44胶质瘤细胞增殖具有明显的抑制作用,并呈剂量依赖性和时间依赖性;倒置显微镜及荧光显微镜下可观察到给药组细胞生长密度低以及出现细胞凋亡的特征性改变;流式细胞仪检测到给药组细胞凋亡比率明显高于空白对照组(P<0.05),并具有剂量依赖性及时间依赖性,同时将胶质瘤细胞阻滞在S期,抑制了细胞周期的进程。结论 As2O3对SHG-44胶质瘤细胞增殖具有明显的抑制作用、可诱导SHG-44胶质瘤细胞的凋亡,并抑制细胞周期进程。Objective To explore the mechanism of proliferative inhibition and apoptosis of arsenic trioxide （ As2O3） on human SHG-44 glioma cells. Methods MTT colorimetric method was applied to detect the proliferative inhibition of As2O3 on glioma cells. The morphologic changes of SHG-44 glioma cells were observed by inverted microscope and fluorescence microscope. Cell apoptosis and interference on cell cycle were detected by FCM. Results As2O3 could inhibit the proliferation of SHG - 44 glioma cells obviously and the inhibitory effect was a dose-dependent and time-dependent tendency. We observed characteristic changes of cell apoptosis and the cells in treatment group grew with low density. FCM shows that apoptosis ceils of the treatment groups were obviously higher than that of control group （ P 〈 0.05 ） in a dose-dependent and time dependent tendency. At the same time,As2O3 could block the cells in S phase and interfer the cell cycle progression. Conclusion As2O3 could inhibit the proliferation of SHG - 44 glioma cells obviously,induce SHG-44 glioma cell apoptosis and interfere the cell cycle progression.

关 键 词：三氧化二砷 神经胶质瘤 细胞凋亡 

分 类 号：R739.4[医药卫生—肿瘤]