姬松茸多糖对染铅大鼠脾脏组织形态及细胞因子mRNA表达的影  

EFFECTS OF AGARICUS BLAZEI MURILL POLYSACCHARIDE ON SPLENIC HISTOMORPHOLOGY AND mRNA EXPRESSION OF THE CYTOKINES IN THE LEAD-POISONING RATS

作　　者：李海杰[1] 冯翠萍[1] 常明昌[1] 程红艳[2] 王晓闻[1] 

机构地区：[1]山西农业大学食品科学与工程学院,太谷030801 [2]山西农业大学资源与环境学院,太谷030801

出　　处：《营养学报》2013年第2期176-180,共5页Acta Nutrimenta Sinica

基　　金：山西省自然科学基金(No.2010011043-1);山西农业大学博士科研启动经费项目(No.412564);山西省科技基础条件平台建设项目(No.2010091017);山西省科技攻关项目(No.021025)

摘　　要：目的研究姬松茸多糖对染铅大鼠脾脏组织形态和相关细胞因子表达的影响,进而探索姬松茸多糖对染铅大鼠的免疫功调节机制。方法选用45日龄SD大鼠,随机分为6组,每组8只,雌雄各半。分别为正常对照组,多糖对照组,染铅模型组、低、中、高剂量姬松茸多糖组(50、100、200 mg/kg.d)。染铅模型组、姬松茸多糖试验组分别给予含0.2%醋酸铅饮水,自由饮用。饲养60 d后,采集脾脏,制备常规HE染色切片,观察姬松茸多糖对染铅大鼠脾脏组织形态的影响,并提取RNA用荧光定量RT-PCR法测定细胞因子核转录因子Kappa B(NF-κB)、细胞间黏附分子-1(ICAM-1)、组织相容性抗原I(MHC-I)mRNA的相对表达量。结果(1)染铅可引起脾脏组织细胞的病理变化,而姬松茸多糖可缓解染铅造成的脾脏损伤。(2)与正常对照组相比,染铅模型组NF-κB、ICAM-1、MHC-ImRNA的表达量分别升高39.6%、67.9%、20.7%,差异显著(P<0.05或P<0.01)。与染铅模型组相比,随着试验组多糖浓度的增加,NF-κB、ICAM-1、MHC-I mRNA的表达量逐渐降低,差异均达到极显著水平(P<0.01)。结论姬松茸多糖能够缓解染铅造成的脾脏损伤,且对染铅大鼠脾脏细胞因子NF-κB、ICAM-1、MHC-I mRNA的高表达有明显的抑制作用,说明姬松茸多糖对染铅大鼠免疫损伤具有保护作用。[营养学报,2013,35(2):176-180]Objective To investigate the immunoregulation activity ofAgaricus blazei Murill polysaccharide （ABMP） in lead-poisoning rats. The effect of ABMP on splenic histomorphology and the mRNA expression of the cytokines in the lead-poisoning rats were studied. Methods Forty-eight healthy SD rats aged 45 d were randomly divided into six groups （female： male=1：1）, control group, polysaccharide group, lead-poisoning model group, low, middle and high dose ABMP groups （50,100 and 200 mg/kg·d）. The lead-poisoning model group and ABMP groups were given 0.2% lead acetate water for free drink. After treatment for 60 d, the spleen samples were collected. Splenic histomorphology were observed with HE stain, and the mRNA expressions of the cytokines, such as nuclear factor κB （NF-κB）, intercellular adhesion molecule （ICAM-1）, class I major histocompatability complex （MHC-I） were assayed with fluorescent quantitative real-time polymerase chain reaction （RT-PCR）. Results （1） Lead-poisoning resulted in cellular changes in spleen. ABMP prevented against the damage of spleen caused by lead. （2） Compared with the control group, the mRNA expression of NF-nB, ICAM-1, MHC-I were significantly up-regulated by 39.6%, 67.9% and 20.7%, respectively in lead-poisoning model group （P〈0.05 or P〈0.01）. Compared with lead-poisoning model group, the mRNA expression of NF-κB, ICAM-1, MHC-I in MBMP groups were significontly decreased （P〈0.01）. Conclusion ABMP can prevent against the damage of spleen by lead and significantly inhibt high mRNA expression of NF-κB, ICAM- 1, MHC-I in the spleen of lead-poisoning rats. ABMP can prevent immune system against the damage induced by the lead-poisoning in rats.

关 键 词：姬松茸多糖 染铅 脾脏组织形态 NF-κB ICAM-l MHC-I 

分 类 号：R285.5[医药卫生—中药学]