HS-SPME-GC-MS联用技术分析杭白菊中挥发性化学成分  

Analysis of Volatile Chemical Components of Dendranthema morifolium by HS-SPME-GC-MS

作　　者：江汉美[1] 张锐[1] 卢金清[1] 李雨玲[1] 杨小金[1] 郭彧[1] 杨珊[1] 

机构地区：[1]湖北中医药大学湖北省药用植物研发中心,武汉430065

出　　处：《中国药房》2013年第19期1784-1786,共3页China Pharmacy

摘　　要：目的:对杭白菊药材中挥发性物质进行分析。方法:采用顶空固相微萃取技术萃取杭白菊中挥发性成分;利用气相色谱-质谱联用技术对其进行定性分析。色谱柱为HP-5MS(50m×0.2mm,0.33μm),载气为氦气,流速为1ml/min,进样口温度为250℃,采用程序升温和不分流进样;电离方式为电轰击电离,电子能量为70eV,离子源温度为230℃,四级杆温度为150℃,接口温度为280℃,质量扫描范围(m/z)为35～550amu;以峰面积归一化法计算各组分的质量分数。结果:共分离出68个峰,确认了其中47个化合物,占挥发油总量的87.11%,其中含量较高的成分有樟脑(10.86%)、菊油环酮(9.03%)、5(-1,1-二甲基乙基)-环戊二烯(8.52%)、α-蒎烯(7.51%)等。结论:试验结果可为杭白菊的进一步研发和综合利用提供科学依据。OBJECTIVE： To analyze the volatile components from Dendranthema morioflium. METHODS： The volatile components was extracted by headspace solid phase micro-extraction （HS-SPME） and qualitatively analyzed by GC-MS. HP-5MS （50 m× 0.2 mm, 0.33 μm） column was used with nitrogen as carder gas at the flow rate of 1 ml/min. Injector temperature was at 250 ℃ through processing temperature rising using splitless inlet; Mass spectrometer conditions were：ionization mode：El, electron energy 70 eV, ion source temperature： 230 ℃, quadropole temperature： 150 ℃, interface temperature： 280 ℃, mass scan range： 35-550 amu. The relative contents of each component were calculated with peak area normalization method. RESULTS： A total of 68 peaks were separated and 47 compounds were identified, which accounted for 87.11% of essential oil. The high content of components was as follows： camphor （10.86%） , chrysanthenone （9.03%） , 5-（1, 1-dimethylethyl）-1, 3-cyclopentadiene （8.52%）and a-pinene （7.51%）, etc. CONCLUSIONS： The study can provide scientific reference for further R＆D and comprehensive utilization of D. morifolium.

关 键 词：杭白菊 顶空固相微萃取技术 气相色谱 质谱联用技术 挥发性成分 

分 类 号：R284.1[医药卫生—中药学] R917[医药卫生—中医学]