白鲜皮化学对照品黄柏酮的提取工艺研究  

Extraction technique of obacunone,a chemical reference substance in Baixianpi(Cortex Dictamni)

作　　者：张勉[1] 孟晓伟[1] 余辉攀[1] 刘朋朋[1] 张宏桂[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《北京中医药大学学报》2013年第4期268-271,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：国家重大新药创制项目(No.2009ZX09308-001-1-6)

摘　　要：目的优选出白鲜皮中对照品黄柏酮的最佳提取工艺。方法采用高效液相色谱法测定黄柏酮含量,采用单因素水平考察乙醇溶剂浓度(65%～95%)、料液比(1∶6～1∶12)、提取时间(1～4 h)、提取次数(1～4次)对黄柏酮提取率的影响;分别对乙醇浓度、料液比、提取时间、提取次数等因素,设计四因素、三水平正交试验法,确定黄柏酮最佳的提取工艺。结果在单因素考察中,乙醇的最佳浓度范围是75%～95%,最佳的料液比范围是1∶8～1∶12,最佳的提取时间范围是1～3 h,最佳的提取次数范围是1～3次。正交试验极差结果分析表明,所考察的因素中,对白鲜皮中黄柏酮提取率的影响程度,从大到小依次为:料液比>提取次数>溶剂浓度>提取时间;黄柏酮的最佳提取工艺为用8倍量95%的乙醇提取2次,每次1 h。按照优化后的提取工艺,黄柏酮的提取率为4.192 mg/g。结论本提取工艺可行,具有工艺流程简单、提取率高的优点。Objective To optimize the best extraction technique of obacunone, a chemical reference substance in Baixianpi （Cortex Dictamni）. Methods The content of obacunone was determined by applying HPLC. The influences of ethanol concentration （65% to 95% ）, solid-liquid ratio （ 1：6 to 1 ： 12）, exaction time （1 h to 4 h） and extraction times （1 time to 4 times） on the extraction ratio of obacunone were observed by using single factor test. A four-factor, three-level orthogonal experiment method was designed respectively according to factors of ethanol concentration, solid-liquid ratio, exaction time and extraction times for determining the best extraction technique of obacunone. Results In the single factor test, the best ethanol concentration was from 75 % to 95 %, the best range of solid-liquid ratio was from 1：8 to 1：12, the best range of exaction time was from 1 hour to 3 hours, and the best range of extraction times was from 1 time to 3 times. The range analysis of orthogonal experiment showed that all influence factors were, in a descending order, solid-liquid ratio, extraction times, ethanol concentration and exaction time according to their influence degree. The best extraction technique of obacunone was as follows： 8 times amount of 95% ethanol was used to extract 2 times and each time for one hour in the extraction. The extraction ratio of obacunone was 4. 192 mg/g by this optimized extraction technique. Conclusion This extraction technique is feasible and simple with higher extraction ratio.

关 键 词：对照品 白鲜皮 黄柏酮 提取工艺 

分 类 号：R284.1[医药卫生—中药学]