ω-3多不饱和脂肪酸对内毒素致大鼠急性肺损伤的影响  

Effect of omega-3 polyunsaturated fatty acid on lipopolysaccharide-induced acute lung injury in rats

作　　者：赵艳[2] 柳欣欣[1] 郭丹[3] 王磊[1] 陈平[1] 

机构地区：[1]扬州大学医学院附属江苏省苏北人民医院普外科,225001 [2]扬州大学医学院附属江苏省苏北人民医院医学实验研究中心,225001 [3]中国医学科学院北京协和医学院北京协和医院中心实验室,100730

出　　处：《中华临床营养杂志》2013年第2期83-89,共7页Chinese Journal of Clinical Nutrition

摘　　要：目的观察肠道给予ω-3多不饱和脂肪酸（PUFA）对脂多糖（LPS）诱导的大鼠急性肺损伤模型的作用。方法将58只雄性SD大鼠分为5组：空白对照组（n=10），模型组（各组n=12），ω-3 PUFA高、中、低剂量组（各组n=12）。造模前7d开始，ω-3PUFA高、中、低剂量组分别口服灌胃t｜）-3PUFA1、0．5、0．25g／kg体重，1次／d，连续7d。末次给药后24h，除空白对照组外，其余各组大鼠尾静脉注射LPS（6mg／kg）造成急性肺损伤模型，造模后观察大鼠一般情况。0、6、24h测量大鼠体温。造模24h后眼静脉丛采血检测血常规和血生化指标。大鼠活杀后取左叶肺称量湿重、干重和湿／干重比（W／D），取右叶肺做光学显微镜下病理学观察，计算半定量病理指数（PI）。结果造模24h后，除空白对照组外其他各组均有死亡，各组指标按n=10计算。大鼠注射LPS后均出现蜷卧少动等症状。6h时模型组体温明显高于空白对照组[（37．4±O．27）℃比（35．9±0．05）℃，P：0．00]；ω-3PUFA高、中、低剂量组体温分别为（36．2±0．38）、（36．3±0．30）、（36．3±0．32）℃，均显著低于模型组（P均=0．01）。模型组白细胞、中性粒细胞和淋巴细胞计数均升高，但与其他组比较，差异无统计学意义。模型组血清谷草转氨酶（GOT）和谷丙转氨酶（GPT）水平明显高于空白对照组[（353±235）U／L比（157±55）U／L，P=0．02；（141±103）U／L比（54±23）U／L，P=0．03]，CO-3PUFA高剂量组的GOT和GPT水平低于模型组[（167±94）U／L，P=0．03；（63±57）U／L，P=0．04]。模型组大鼠肺湿重；（371±38）mg比（281±24）mg，P=0．01]和W／D均显著高于空白对照组（7．34±1．40比5．41±0．84，P=0．01）；ω-3 PUFA高、中、低剂量组W／D较模型组显著降低（6．17±0．58，P=O．03；6．17±O．76，P=0．03；6．13±1．23，P=0．04）。光学显微镜下观Objective To evaluate the effect of dietary ω-3 polyunsaturated fatty acid （PUFA） supple- mentation on lipopolysaccharide （LPS）-indueed acute lung injury in rats. Methods Totally 58 male SD rats were divided into control group （ n = 10）, model group （ n = 12）, ω-3 PUFA high-dose group （ n = 12 ）, ω-3PUFA medium-dose group （n = 12）, and ω-3 PUFA low-dose group （n = 12）. Seven days before model estab- lishment, rats in the three ω-3PUFA groups were orally given ω-3 PUFA at 1, 0. 5, and O. 25 g/kg body weight once per day, respectively, for seven consecutive days. Twenty-four hours after the last administration, all rats except those in the control group were given intravenous injection of LPS （6 mg/kg） at caudal vein to establish the model of acute lung injury. Body temperature was measured at O, 6, and 24 hour. Blood samples were collected from the eye venous plexus for routine blood tests and blood biochemical tests 24 hours after mod- eling. After the rats were sacrificed, the left lung was harvested for measuring the wet weight and dry weight and calculating the wet/dry weight ratio （W/D）. The right lung was harvested for pathological observation un- der light microscope and calculation of semi-quantitative pathological index （PI）. Results Twenty-four hours after modeling, deaths were noted in all groups except the control group. After injection of LPS, rats curled with little movements. At 6 hour, the body temperature was significantly higher in the model group than in the con- trol group [ （37.4 ±0. 27） ℃ vs. （35.9 ±0. 05） ℃, P =0.00] ; it was （36. 2 ±20. 38） ℃, （36. 3 ±20. 30） ℃, and （ 36. 3 ± 0. 32） ℃ in the ω-3 PUFA high-, medium-, and low-dose groups, which were significantly lower than that in the model group （all P =0. 01 ）. The amounts of white blood cells, neutrophils, and lymphocytes increased in the model group, but showing no significant difference compared with the other groups. The serum glutamic oxalacetic tr

关 键 词：Ω-3多不饱和脂肪酸 二十二碳六烯酸 二十碳五烯酸 脂多糖 急性肺损伤 

分 类 号：R563.8[医药卫生—呼吸系统]