去铁敏干预蛛网膜下腔出血后溶酶体组织蛋白酶表达研究  

Desferrioxamine(DFO) Reduces the Expression of Cathepsin B and Cathepsin D in Rats Earlier Injured Brain of Subarachnoid Hemorrhage

作　　者：贾阳[1] 虞正权[1] 陈罡[1] 陈传新[1] 刘泽昊[1] 

机构地区：[1]苏州大学附属第一医院神经外科,江苏苏州215006

出　　处：《现代生物医学进展》2013年第6期1026-1029,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金青年科学基金项目(81100872)

摘　　要：目的:探讨大鼠蛛网膜下腔出血(SAH,Subarachnoid Hemorrhage)早期脑损伤中溶酶体组织蛋白酶B(Cathepsin B)、溶酶体组织蛋白酶D(Cathepsin D)的表达变化及去铁敏(DFO)对其的影响。方法:将24只SD大鼠随机分为四组:正常对照组(6只),SAH模型组(6只),安慰剂组(6只),DFO药物组(6只),视交叉前池注血法(APC)制作大鼠SAH模型,免疫组化分别检测Cathepsin D、Cathepsin B的蛋白表达,干湿法测定48 h脑含水量。结果:与正常组比较,SAH模型组大鼠48 h后Cathepsin D、Cathepsin B的蛋白表达明显增强,水肿指数增高,DFO药物组大鼠48h后Cathepsin D、Cathepsin B的蛋白表达较SAH组明显减低,水肿指数减低。结论:Cathepsin D、Cathepsin B在大鼠蛛网膜下腔出血后表达增强,DFO能减少其表达并对早期脑损伤有保护作用,溶酶体可能参与了蛛网膜下腔出血早期脑损伤的过程,稳定溶酶体膜,减少Cathepsin B/D的释放可能为SAH后早期脑损伤提供新的治疗途径。Objective： To study the expression of Cathepsin B and Cathepsin D in rats early brain injury after subarachnoid hemor-rhage and the effect of desferrioxamine （DFO） on SAH. Methods： 24 SD rats were randomly divided into four groups： normal group （n=6）, placebo group （n=6）, DFO group （n=6） and SAH group （n=6）. SAH group was treated with pre-chiasmatic cistern （APC） autolo-gous blood injection, DFO group were treated with DFO after pre-chiasmatic cistern （APC） autologous blood injection. The expression of Cathepsin B and Cathepsin D were evaluated by immunohistochemistry. Results： Compared with normal group, the expression level of Cathepsin B and Cathepsin D increased significantly in SAH group, rather Cathepsin B and Cathepsin D reduced when treated with DFO. Conclusions： The expression of Cathepsin B and Cathepsin D increased after 48 hours of subarachnoid hemorrhage, DFO could inhibit the expression of Cathepsin B and Cathepsin D and relieve early brain injury. Lysosomes may be involved in the process of earlier injured brain of rats after subarachnoid hemorrhage, stabilizing lysosome membrane and reducing the release of protease may provide a new ther-apeutic approach in early brain injury after SAH.

关 键 词：蛛网膜下腔出血 溶酶体 CATHEPSIN D CATHEPSIN B 去铁敏desferrioxamine(DFO) 

分 类 号：Q95-3[生物学—动物学] R743.35[医药卫生—神经病学与精神病学]