MXRA5在结直肠癌中的表达及其意义  

Analysis of Clinical Pathological Features of Colorectal Mucinous Adenocarcinoma

作　　者：王光辉[1] 姚玲[1] 汤文涛[1] 高军[1] 杜鹏[1] 崔龙[1] 

机构地区：[1]上海交通大学附属新华医院肛肠外科,上海200092

出　　处：《现代生物医学进展》2013年第6期1033-1037,1078,共6页Progress in Modern Biomedicine

基　　金：上海交通大学医学院科技基金项目-甲基化对Hedgehog信号通路调节在黏液性大肠癌及其亚型中的作用(YZ1038)

摘　　要：目的:在我们的前期实验中,我们运用质谱技术研究新鲜培养的结直肠癌和对应的正常黏膜蛋白组学差异时发现基质重建相关蛋白5(MXRA5)在两者之间的表达有明显的差异,而目前并没有关于MXRA5是否可以作为结直肠癌肿瘤标志物的研究;本实验的目的是探索MXRA5在结直肠癌中的表达情况以及其临床意义。方法:运用免疫组化和实时定量PCR技术,对MXRA5在结直肠中的表达情况进行检测,并分析其与临床病理特征的关系。结果:MXRA5在结直肠正常组织、腺瘤和肿瘤中的免疫评分有明显差异(P<0.05),而且MXRA5的阳性表达与肿瘤的分期和大网膜转移明显相关。基因水平MXRA5的表达没有发现明显差异。结论:我们的研究结果证实了MXRA5在肠癌组织中存在异常表达,并且可能是结直肠癌早期诊断或者大网膜转移的肿瘤标志物。Objective： In our previous study, a significant highly-expressed level of matrix-remodelling associated 5 （MXRA5） was found in flesh cultured colorectal cancer （CRC） tissues compared with their normal adjacent mucosa by differential secretome analysis. Whether MXRA5 was a potential tissue biomarker of CRC has not been evaluated. The aim of this study is to investigate the association between MXRA5 expression and clinicopathological characteristics of CRC patients. Methods： The MXRA5 expression levels were determined by qRT-PCR and immunohistochemistry. Relative quantity （RQ） value and irrmaunoreactive score（IRS） were used for quantitative assessment. Results： There were significant difference of the MXRA5 expression in CRC tissues and Adenoma tissues compared with Normal tissues （P〈0.05）. However, MXRA5 mRNA expression levels showed no significant differences between CRC tissues and their corresponding normal tissues. Conclusion： Our revealed reveal that MXRA5 protein expression is aberrantly detected in CRC tissues, and has the potential value of biomarker for CRC early detection and omentum metastasis prediction.

关 键 词：结直肠肿瘤 蛋白质组学 病理学 临床 

分 类 号：R735.3[医药卫生—肿瘤]