腹腔感染大鼠肠粘膜通透性改变与I-FABP表达关系  

Study on Correlation between Intestinal Permeability and I-fabp in Plasma in Rats with Intraperitoneal Infection

作　　者：赵延东[1] 丁连安[2] 姚如永[2] 刘路光[2] 侯钦猛[2] 

机构地区：[1]青岛大学医学院普外科,山东青岛266003 [2]青岛大学医学院附属医院,山东青岛266003

出　　处：《现代生物医学进展》2013年第6期1045-1047,共3页Progress in Modern Biomedicine

基　　金：山东省自然科学基金项目(ZR2010HM124)

摘　　要：目的:观察腹腔感染大鼠肠屏障损伤后肠道通透性的改变与I-FABP表达的关系。方法:清洁级健康的成年雄性Wistar大鼠40只,随机分为空白对照组(仅行简单的剖腹手术)和腹腔感染(采用CLP盲肠结扎穿孔法制作腹腔感染模型)术后12 h、24 h、36h、48 h组,每组8只,相应时间点处死大鼠后,测定血浆D-乳酸和I-FABP含量。结果:腹腔感染后12 h,大鼠血浆D-乳酸含量开始增加(P<0.05),血浆I-FABP含量也开始增加(P<0.01);血浆D-乳酸含量在24 h达到最高值(P<0.01),血浆I-FABP含量在36h达到最高值(P<0.01)。大鼠血浆D-乳酸水平与I-FABP具有显著相关性(r=0.626,P<0.01)。结论:腹腔感染状态下,肠屏障严重受损,肠粘膜通透性的改变与血浆I-FABP水平具有显著的相关性。Objective： To observe the correlation between the intestinal permeability with I-FABP in plasma after gut barrier injury in rats with intraperitoneal infection. Methods： Forty healthy adult Wistar rats were randomly divided into control group （celiotomy ones only） and groups of 12, 24, 36, 48 hours post-operation of abdominal infection respectively （These groups were treated by cecal ligation and puncture to establish abdominal infection models）. Each group contained 8 rats. After the rats were anaesthetized, D-lactate and I-FABP in plasma were measured at 4 consecutive time points. Results： Intestinal barrier began to change significantly 12 h after the in-fection, The lever of D-lactate in plasma（P〈0.05） is higher than the control group, and reached the peak at 24h postoperation（P〈0.01）. Meanwhile the level of I-FABP in plasma（P〈0.01） was higher than that of the control group, and reached the peak at 36 h postoperation （P〈0.01）. D-lactate level also had a significant correlation with I-FABP level （r=0.626, P〈0.01）. Conclusion： Intestinal mucosal barrier was subjected serious damage and there was a significant correlation between intestinal permeability and I-FABP level in plasma in rats with intraperitoneal infection.

关 键 词：腹腔感染 肠屏障 肠通透性 D-乳酸 I-FABP 

分 类 号：Q95-33[生物学—动物学] R574[医药卫生—消化系统]