应用基因芯片技术研究原发性腹膜后脂肪肉瘤组织特异性候选基因  

Analysis of Tissue-specific Candidate Genes in Primary Retroperitoneal Liposarcoma by Gene Chips

作　　者：李伟民[1] 李霄[1] 周景师[1] 李俊杰[1] 李琳[1] 韩玮[1] 王建峰[1] 姜楠[1] 于泳[1] 张卓超[1] 王德盛[1] 窦科峰[1] 

机构地区：[1]第四军医大学西京医院肝胆胰脾外科,陕西西安710032

出　　处：《现代生物医学进展》2013年第7期1205-1209,1218,共6页Progress in Modern Biomedicine

基　　金：国家高科技研究发展计划"863计划"(2012AA021005)

摘　　要：目的:原发性腹膜后脂肪肉瘤是一种少见的恶性肿瘤,发病率大约占全部恶性肿瘤的1%,但其却是腹膜后最常见的软组织肿瘤。通过研究原发性腹膜后脂肪肉瘤和正常脂肪组织间的基因表达差异,可以寻找到与原发性腹膜后脂肪肉瘤发生、发展相关的候选基因并为其防治研究提供线索。方法:应用基因芯片技术检测2例原发性腹膜后脂肪肉瘤和相应的正常脂肪组织全基因组序列,分析其基因表达差异。结果:两组间的差异表达基因3828个,其中表达上调有1837个基因,表达下调有1991个基因。其中涉及细胞增殖、黏附、凋亡以及信号通路等多种生物学过程。结论:通过基因表达谱芯片筛选出与原发性腹膜后脂肪肉瘤发生和进展密切相关的基因为其基础研究及临床早期诊断、预防和治疗提供潜在的分子标记和靶基因。Objective： Primary retroperitoneal liposarcoma is a kind of rare tumor that represents approximately 1% of all diagnosed malignant neoplasms, but it is the most frequent histopathological variety of the retroperitoneum. To find Candidate genes in the development of primary retroperitoneal liposarcoma, and to provide clues for prevention and cure research, the differential expressions of genes between primary retroperitoneal liposarcoma and normal adipose tissue were investigated. Methods： The application of gene chip technology test two cases of primary retroperitoneal liposarcoma and corresponding normal adipose tissue of the whole genome sequence, analyzed the differences of gene expression. Results： A total of 3828 genes were differentially expressed in the two groups, including 1837 up-regulated genes and 1991 down-regulated genes, including the genes associated with cell proliferation, adhesion, apoptosis and signaling pathway and so on. Conclusions： Microarray analysis was used to detect the expression of genes related to the origin and development of primary retroperitoneal liposarcoma, because of its basic research and clinical early diagnosis, the results also provided molecular and target genes for its prevention and treatment.

关 键 词：基因芯片 候选基因 原发性腹膜后脂肪肉瘤 脂肪组织 

分 类 号：R735.4[医药卫生—肿瘤] Q75[医药卫生—临床医学]