TGF-β_1在应力介导的牙周膜成纤维细胞增殖中的作用及其机制  

The Effect of TGF-β_1 on Proliferation by Cultured Periodontal Ligament Fibroblasts under Stress

作　　者：薛敏[1] 于江波[1] 张月[1] 袁晓[1] 张广耘[1] 贾萍萍[1] 刘丽娟[1] 孙仙蕊[1] 

机构地区：[1]青岛大学医学院附属青岛市立医院口腔科,山东青岛266071

出　　处：《现代生物医学进展》2013年第7期1240-1243,1344,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(31170891);青岛市卫生局科研基金项目(2010-WSZD009)

摘　　要：目的:探讨周期性张应力作用下人牙周膜成纤维细胞(HPDLF)转化生长因子β(1TGF-β1)对其细胞增殖的作用,及对其I型胶原基因表达的作用和影响。方法:在成功构建人牙周膜成纤维细胞体外培养力学刺激模型的基础上,利用多通道细胞牵张应力加载系统,对细胞分别施加2、6、12与24 h的周期性张应力,以不加力组为对照组,观察各组细胞形态变化,利用细胞计数试剂8检测细胞增殖活性,并利用ELISA试剂盒检测加力后各组TGF-β1的表达,并对加力12 h组加入TGF-β1抑制剂SB431542,利用RT-PCR检测技术检测对I型胶原基因表达的影响。结果:与对照组比较,加力2 h细胞增殖稍降低,6 h增殖活性增强,12 h达到峰值,24 h增殖活性明显受到抑制;TGF-β1的表达与细胞增殖成正相关;TGF-β1受到抑制后细胞增殖受到影响,I型胶原的表达也受到影响。结论:人牙周膜成纤维细胞的增殖在一定时间的周期性张应力作用下先增加然后再抑制,其中TGF-β1参与细胞增殖,并且TGF-β1对人牙周膜成纤维细胞I型胶原的表达起促进作用。ABSTRACT Objective： To investigate the effects of transforming growth factor β1 （TGF-β1） secreted by human periodontal ligament fibroblast （HPDLF） on cell proliferation and collagen gene expression under the cyclical tensile stress. Methods： On the basis of successfully constructed the human periodontal ligament fibroblasts cultured in vitro and mechanical stimulation model, cyclic stretch was applied on the fibroblasts for 2,6,12 and 24 hours by multi-channel cells distraction stress loading system. The group without cyclic stretch was regarded as the control group. The cellular proliferation was measured with CCK-8 and the expression of TGF-β1 was measured with ELISA Kits. Then 12 hour group was added TGF-β1 inhibitor （SB431542） and measured the gene expression with RT-PCR detection techniques. Results： Compared with the control group, the proliferation activity of HPDLF decreased at 2 h, rised at 6h, reached the peak at 12 h, and was significantly inhibited at 24 h. There was positive correlation between expression of TGF-β1 and the cell proliferation. The cellular proliferation and expression of type I collagen were affected when TGF-β1 was inhibited. Conclusions： In a certain time, HPDLF proliferation was promoted within the scope of cyclic stretch, then obviously inhibited as time went by. TGF-β1, which involved in cellular proliferation, played a catalytic role in the expression of type I collagen of HPDLF.

关 键 词：人牙周膜成纤维细胞 周期性张应力 TGF-β1 细胞增殖 I型胶原 

分 类 号：R781.4[医药卫生—口腔医学] R318.01[医药卫生—临床医学]