温郁金抗炎抗氧化活性内生真菌的筛选  

Screening of endophytic fungi from Curcuma wenyujin with anti-inflammatory and anti-oxidant activities

作　　者：王艳红[1] 朱艳萍[1] 杨信东[2] 吴晓民[3] 

机构地区：[1]吉林农业大学农业部参茸产品质量监督检验测试中心,吉林长春130118 [2]吉林农业大学农学院,吉林长春130118 [3]吉林农业大学附属医院,吉林长春130118

出　　处：《药物评价研究》2013年第2期90-94,共5页Drug Evaluation Research

基　　金：吉林农业大学科研启动基金资助项目(201110)

摘　　要：目的研究温郁金内生真菌的抗炎抗氧化活性,旨在获得有良好抗炎抗氧化活性的菌株,以寻找新型抗炎抗氧化活性物质。方法采用二甲苯致小鼠耳廓肿胀及醋酸致小鼠腹腔毛细血管通透性的动物模型对已分离的50株温郁金内生真菌发酵产物进行抗炎活性筛选;采用DPPH法测试内生真菌的抗氧化活性。结果有21株内生真菌能够抑制二甲苯诱导的小鼠耳肿胀,并且对醋酸所致小鼠腹腔毛细血管通透性有降低作用,其中菌株E3、E8、E17、E24、E37、E46的抑制作用最为显著(P<0.01),作用与萘普生相当;具有抗氧化活性的菌株8株。并且其抗氧化活性存在剂量效应关系,其中活性菌株E2、E17、E37在质量浓度为140μg/mL时对DPPH自由基清除率都在90%以上。结论温郁金含有抗炎抗氧化活性的内生真菌,菌株E2、E3、E8、E17、E24、E37、E46具有良好的抗炎抗氧化效果,具有一定的研究开发前景。Objective To study the anti-inflammatory and anti-oxidant activities of endophytic fungi from medicinal plant Curcuma wenyujin,to obtain the strains with good anti-inflammatory and anti-oxidant activities,and to find the new substances with anti-inflammatory and anti-oxidant activities.Methods The anti-inflammatory activities of 50 strains from the isolated endophytic fungi were tested by animal models with ear swelling induced by xylene and peritoneal capillary permeability induced by acetic acid in mice;DPPH method was used to evaluate the anti-oxidant activities of endophytic fungi.Results Twenty-one strains of endophytic fungi could inhibit ear swell induced by xylene in mice and reduce peritoneal capillary permeability of mice induced by acetic acid.Compared with the group of physiological saline,the groups of E3,E8,E17,E24,E37,and E4 had the significant inhibitory effects(P< 0.01),which was the same as Naproxen;Eight strains of endophytic fungi had the anti-oxidant activity.There was a dose-effect relationship between the fermentation products and the anti-oxidant activity,and their free radical scavenging rates against DPPH were above 90% when the concentration of E2,E17,and E37 were at 140 μg/mL.Conclusion Some endophytic fungi from C.wenyujin have the anti-inflammatory and anti-oxidant activities,strains E2,E3,E8,E17,E24,E37,and E46 show the significant effect on the anti-inflammatory and anti-oxidant activities,which has a perspective in research and development.

关 键 词：温郁金 内生真菌 抗炎活性 抗氧化活性 

分 类 号：R965.1[医药卫生—药理学] R971.1[医药卫生—药学]