我国近期分离的6株狂犬病病毒的糖蛋白基因序列分析  

Analysis on glycoprotein gene sequences of 6 representative rabies viruses recently isolated in China

作　　者：黄思佳[1] 解庭波[1] 吴杰[1] 王月[1] 季雅琦[1] 严家新[1] 徐葛林[1] 

机构地区：[1]武汉生物制品研究所有限责任公司基因工程室,430060

出　　处：《国际生物制品学杂志》2013年第2期57-60,共4页International Journal of Biologicals

基　　金：基金项目：国家高技术研究发展计划（2007AA022402）

摘　　要：目的分析我国近期分离的6株代表性狂犬病病毒（rabiesvirus，RV）的糖（G）蛋白基因序列，了解RV街毒株与疫苗株在核苷酸和氨基酸序列水平的差异。方法对6个RV街毒株G基因进行逆转录和PCR扩增，经纯化、克隆、测序后获得6条G基因全长序列，采用生物信息学软件对G基因序列进行分析。结果6个街毒株的G蛋白在核苷酸水平的同源性为97．7％－100％，在氨基酸水平的同源性为97．9％－100％；与CTN－181疫苗株的核苷酸序列同源性最高，为86．7％－87．2％，氨基酸序列同源性为92．7％～93．1％。结论6个RV街毒株的G蛋白在核苷酸及氨基酸水平上均有变异，但与CTN．181疫苗株关系最近，表明CTN－181疫苗株可能为我国境内流行的狂犬病提供良好的预防作用。Objective To analyze glycoprotein （G） gene sequences of 6 representative rabies viruses recently isolated in China and understand the differences between the street virus strains and vaccine strains at the level of nucleotide and amino acid sequences. Methods The G genes were amplified by PCR and sequenced. The nucleotide and amino acid sequences were analyzed with bioinformatics software. Results The nucleotide sequence homologies of G genes from the 6 street strains were 97.7% -100%, and the deduced amino acid identities were 97.9% -100%. The similarities between these isolates and CTN-181 vaccine strain were 86.7%-87.2% and 92.7%-93.1% at nucleotide and amino acid level, respectively. Conclusions Mutations in both gene sequences and deduced amino acid sequences are found in G proteins of the 6 street virus strains, but these strains are much closer to CTN-181 vaccine strain than other vaccine strains.

关 键 词：狂犬病病毒 糖蛋白类 基因型 序列分析 DNA 

分 类 号：R373[医药卫生—病原生物学]