重组人胰岛素样生长因子-1工程菌发酵培养基的优化  

Optimization of Fermentation Medium for Recombinant Human Insulin-Like Growth Factor-1 Engineering Bacteria

作　　者：杨渐[1] 陈理[1] 俞昌喜[1] 

机构地区：[1]福建医科大学药学院药理学系,福建福州350001

出　　处：《化学与生物工程》2013年第4期36-40,共5页Chemistry & Bioengineering

基　　金：福建省高校产学合作科技重大项目(2010Y4002)

摘　　要：在摇瓶条件下,以M9培养基为基础,采用单因素实验和正交实验,对适合于表达重组人胰岛素样生长因子-1工程菌生长的高密度发酵培养基进行了优化。确定最佳培养基配方为:葡萄糖0.4%、甘油1.5%、酵母粉3%、胰蛋白胨3%、Na2HPO41.2%、KH2PO40.6%、NH4Cl 0.1%、NaCl 0.20%、MgSO40.048%,在此优化培养基中培养14h,工程菌菌体密度OD600达7.5。In order to obtain the suitable fermentation medium for high cell density cultivation of engineer- ing Escherichia coli with the expression of human insulin-like growth factor-I, carbon sources,nitrogen sources and inorganic salts in M9 medium were optimized by single factor experiment and orthogonal experiment in shake-flask fermentation. The results showed that the optimal fermentation medium composition were as fol- lows： glucose 0. 4%, glycerol 1.5 %, yeast powder 3 %, tryptone 3 %, Na2 HPO4 1.2%, KH2 PO4 0. 6 %, NH4 Cl 0. 1%, NaCl 0.20 %, MgSO4 0.048 %. And cell density OD600 of the engineering bacteria reached 7.5 when cul- tured 14 h in the above optimal medium.

关 键 词：人胰岛素样生长因子-1 大肠杆菌 发酵培养基 优化 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]