超级黑糯玉米芯色素的抗肿瘤和体外抗氧化作用  

Anti-Cancer Effect and Antioxidant Activity of Super Black Glutinous Corncob Pigment

作　　者：冉颖霞[1] 宫坤[2] 周杨[1] 张庭廷[1] 

机构地区：[1]安徽师范大学生命科学学院生物环境与生态安全安徽省高校重点实验室,安徽芜湖241000 [2]淮北普豪生物科技有限公司,安徽濉溪235100

出　　处：《安徽师范大学学报（自然科学版）》2012年第4期351-354,359,共5页Journal of Anhui Normal University(Natural Science)

基　　金：国家自然科学基金(30870429);安徽师范大学科学研究重点项目(2009xzd07)

摘　　要：为研究超级黑糯玉米芯色素(super black glutinous corncob pigment,SBCP)对肿瘤细胞的增殖抑制效应和体外抗氧化活性.采用MTT法检测了SBCP对人肝癌细胞系BEL-7402以及胃癌细胞系SGC-7901增殖的影响;在体外化学模拟的条件下,通过建立DPPH自由基、羟基自由基、超氧阴离子自由基、还原力试验,采用比色法测定对比了SBCP和抗坏血酸(Vc)对DPPH自由基、羟基自由基和超氧阴离子的清除作用以及还原能力.结果表明SBCP对BEL-7402细胞和SGC-7901细胞增殖有明显的抑制作用,且随药物浓度的增大而增强;SBCP对两种肿瘤细胞的半数抑制浓度(IC50)分别为4.13 mg/mL和3.83 mg/mL.SBCP还具有较好的抗氧化作用,对羟基自由基和超氧阴离子的清除作用分别是Vc的1.4倍和1.23倍,还原能力是Vc的1.77倍,但对DPPH自由基的清除作用略低于Vc.SBCP在癌症预防和治疗以及食品保健方面具有潜在的应用价值;SBCP具有很好的体外抗氧化活性.In order to research the inhibitory effect on cancer cell and in vitro antioxidant activity of super black glutinous corncob pigment （SBCP）. The growth inhibition of SBCP on human hepatocellular carcinoma cell line BEL-7402 and gastric cancer cell line SCC- 7901 in vitro were measured by MTT assay. In vitro chemical simulated systems, the abilities of SBCP to scavenge DPPH radical, hydroxyl radical, superoxide anion radical as well as reducing power were tested using colorimetric methods, and compared with aseorbic acid （Vc）. Results showed that SBCP had significant inhibitory effect on proliferation of the BEL - 7402 cells and SGC - 7901 cells, and the inhibition increasing with the increase of SBCP concentration. ICS0 of SBCP BEL - 7402 and SGC- 7901 were respective 4. 13mg/mL and 3.83mg/mL. SBCP also had obviously antioxidant effect. Compared with the effects of Vc, the abilities to scavenge hydroxyl radical, superoxide anion radical as well as reducing power of SBCP were 1.40, 1.23 and 1.77 times than that of Vc respectivily, only the ability of scavenge DPPH radical was lower than Vc. Conclusions SBCP has potentially application value in cancer prevent and therapy as well as health food. SBCP has good antioxidant activity in vitro.

关 键 词：超级黑糯玉米芯色素 BEL-7402细胞 SGC-7901细胞 MTT 抗氧化 

分 类 号：TS202.3[轻工技术与工程—食品科学]