内蒙古地区结核分枝杆菌串联重复序列基因多态性分析  

Polymorphism of variable number tandem repeat gene of Mycobacterium tuberculosis isolates from Inner Mongolia

作　　者：苏云开[1] 余琴[2,3] 吕冰[2] 马岩[3] 连璐璐[2,3] 杨晓敏[1] 董海燕[2] 刘耀[1] 赵秀芹[2] 吴移谋[3] 万康林[2,3] 

机构地区：[1]内蒙古自治区结核病防治所,内蒙古呼和浩特010010 [2]中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室 [3]南华大学病原生物学研究所

出　　处：《疾病监测》2013年第4期260-264,共5页Disease Surveillance

基　　金：国家科技重大专项(No.2008ZX100/03-010-02;2011ZX10004-001);传染病预防控制国家重点实验室项目(No.2011SKLID208)~~

摘　　要：目的了解内蒙古地区结核分枝杆菌临床分离株的串联重复序列(variable number tandem repeat,VNTR)基因多态性和VNTR基因型构成,及不同VNTR位点在该地区结核分枝杆菌基因分型中的应用。方法从内蒙古自治区结核病防治研究所收集临床分离的结核分枝杆菌菌株及其病例背景资料,采用多位点串联重复序列分析(MLVA)对收集的菌株进行22个VNTR位点分析,计算Hunter-Gaston指数(HGDI),分析各个位点的分辨率,同时用BioNumerics软件对VNTR结果进行聚类分析。且统计学分析民族与主要基因型间的关系。结果 372株菌共分为308个基因型,47簇,261个独特基因型,成簇率为17.20%。22个VNTR位点的基因多态性存在较大的差异,位点VNTR3820(HGDI 0.838)的分型分辨能力最高,MIRU23(HGDI 0.068)和MIRU27(HGDI 0.083)分型分辨能力较差。随着VNTR位点的增加,分型的分辨能力也有所提高。Ⅰ群基因型菌株与民族易感性的分析表明,汉族与蒙古族间Ⅰ群基因型菌株分布差异无统计学意义(χ2=0.337,P=0.561>0.05)。结论内蒙古地区结核分枝杆菌临床分离株基因具有多态性,不同VNTR位点在内蒙古地区结核分枝杆菌中具有不同的分辨能力。且Ⅰ群基因型为该地区主要流行株,而Ⅰ群基因型菌株与民族易感性间无关联。Objective To understand the polymorphism and proportion of variable number tandem repeat （VNTR） gene of Mycobacterium tuberculosis isolates from Inner Mongolia and the application of different VNTR loci for M. tuberculosis genotyping in Inner Mongolia. Methods The clinical M. tuberculosis strains isolated from confirmed tuberculosis patients, including the patients＇ epidemiological and clinical information, were collected from Inner Mongolia Institute for Tuberculosis Control and Prevention and analyzed by the way of multi-Locus VNTR analysis （MLVA） with 22 VNTR loci. The polymorphism of different 22 VNTR loci were analyzed by the Hunter-Gaston Index with Bionumerics 5.0 software. Results Of all the 372 strains, 308 genotypes with 22 VNTR loci were detected, including 47 genotype clustering and 261 distinct genotypes. The clustering rate of genotype was 17.20%. In addition, the 22 loci had different polymorphisms, VNTR3820 （ HGDI 0. 838 ） locus had highest discriminatory power, whereas MIRU23 （ HGDI 0. 068 ） and MIRU27 （ HGDI 0. 083 ） loci had lower discriminatory power. With the increase of the number of VNTR loci, the discriminatory power of VNTR analysis for M. tuberculosis strains was improved. Conclusion Gene polymorphism of clinical M. tuberculosis strains was observed in Inner Mongolia, and the different loci had distinct discriminatory oower in VNTR analysis

关 键 词：结核分枝杆菌 可变数目串联重复序列 基因多态性 多位点串联重复序列分析 

分 类 号：R378[医药卫生—病原生物学]