HPLC法同时检测水产品中残留氯霉素、四环素、金霉素和土霉素  

HPLC method for simultaneous detection of chloramphenicol,tetracycline,chlortetracycline and oxytetracycline in aquatic products

作　　者：许韡 吴云[1] 

机构地区：[1]上海市虹口区疾病预防控制中心,上海200082

出　　处：《中国卫生检验杂志》2013年第4期819-820,823,共3页Chinese Journal of Health Laboratory Technology

基　　金：上海市虹口区疾病预防控制中心人才培养计划(jk2010-05)

摘　　要：目的:建立水产品中氯霉素、四环素、土霉素、金霉素残留的高效液相色谱法同时检测方法。方法:样品(有鳍鱼、虾、蟹、贝壳类)经5%高氯酸溶液沉淀蛋白质,以HPLC同时检测其中四种抗生素含量,色谱柱:ZORBAX SB-C18 4.6×250 mm 5μm,流动相:甲醇+乙腈+0.01 mol/L草酸溶液梯度洗脱,流速1.0 ml/min,检测波长270 nm。结果:本法四种抗生素的最低检出浓度均为0.01 mg/kg,在0.01 mg/kg～1 mg/kg范围内线性良好,加标回收率96.0%～102%,RSD<1.0%(n=6)。结论:该法具有准确、灵敏、简便等优点,适用于同时检测水产品中上述四种抗生素。Objective：To establish a HPLC method for the simultaneous detection of chloramphenicol,tetracycline,oxytetracycline,chlortetracycline residue in aquatic products.Methods： Finfish,shrimp,crabs,shellfish were grounded and 5% perchloric acid was added to precipitate protein,and finally analyzed with HPLC.The chromatographic column was ZORBAX SB-C18 4.6 ×250 mm 5 μm,mobile phase was methanol and acetonitrile ＋0.01 mol/L oxalic acid solution in gradient elution mode,the flow rate was 1.0 ml/min,the detection wavelength was 270 nm.Results： The limit of detection for the four antibiotics were 0.01 mg/kg.In the range of 0.01 mg/kg^1 mg/kg,it had a good linearity with recovery rate of 96.0%~102% and RSD1.0%（n=6）.Conclusion： The method is accurate,sensitive,simple,and it is suitable for simultaneous detection of the four antibiotics in the aquatic products.

关 键 词：氯霉素 四环素 土霉素 金霉素 水产品 高效液相色谱法 

分 类 号：O657.72[理学—分析化学]