抑癌基因P16在喉癌中的表达  

Expression of tumor suppressor gene P16 in laryngeal cancer

作　　者：万福生[1] 曾昭建[1] 张少容[2] 万义福[3] 

机构地区：[1]江西医学院生化与分子生物学教研室,南昌330006 [2]江西医学院第二附属医院 [3]江西省肺科医院

出　　处：《中国肿瘤临床与康复》2000年第5期32-33,共2页Chinese Journal of Clinical Oncology and Rehabilitation

摘　　要：目的　研究抑癌基因P16与喉癌的关系。方法　用免疫组化和原位杂交方法检测 18例喉癌组织中抑癌基因P16蛋白及mRNA的表达。结果　P16蛋白在喉癌、交界性肿瘤及正常喉粘膜中的检出率分别为 2 2 .2 2 %、44.44%及 6 6 .6 7% ,原位杂交表明P16mRNA在上述组织中的阳性率分别为 2 7.78%、5 5 .5 6 %及 83 .33 % ,各组分别与喉癌相比 ,均有显著性差异 (P <0 .0 5 )。并显示P16表达阳性率随喉癌病理分级的上升而降低。结论　P16在喉癌中的表达明显低于正常喉粘膜组织 ,它与喉癌的发生发展密切相关 ,可作为喉癌生物学行为的评估指标。Objective To observe the relationship between tumor suppressor gene P16 expression and laryngeal cancer.Methods Immunohistochemistry and in situ hybridization were used to study the expression of P16 in 18 cases of laryngeal cencer.Results The positive rate of P16 protein in laryngeal cancer,borderline to tumor and normal laryngeal tissue were 22.22%,44.44% and 66.66% respectively(P<0.05).while the positive rate of P16 mRNA of in situ hybridization in the order mentioned above were 27.78%,55.56% and 83.33% respectively(P<0.05).Conclusions The positive rate of P16 protein and mRNA in laryngeal cancer is significant lower than that in borderline of tumor and normal laryngeal tissue.P16 is an important tumor suppressor gene,the mutation and deletion of P16 are closely related with the genesis and development of larnygeal cancer. [

关 键 词：喉肿瘤 P16抑癌基因 原位杂交 免疫组织化学 

分 类 号：R739.6[医药卫生—肿瘤]