不同处理技术对环境扫描电镜下细菌原始形态的影响  

The impact of different processing techniques on the original form of environmental scanning electron microscope bacteria

作　　者：孙镇平[1] 李佳[2] 刘洪红[2] 王忠敏[1] 孙伟[2] 李敬盼[2] 单哲[2] 薛太平[3] 顾玉泉 

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]扬州大学动物科学与技术学院,江苏扬州225009 [3]泰兴市畜牧兽医中心,江苏泰兴225400

出　　处：《扬州大学学报（农业与生命科学版）》2013年第1期41-43,共3页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：江苏省科技厅前瞻性高新技术研究项目(BY2011138);江苏高校优势学科建设工程资助项目

摘　　要：应用常规高真空扫描电镜观察生物样品必须经过干燥和导电处理,但无论是采用冷冻干燥还是临界点干燥,都存在着样品不同程度脱水失真的问题,很多生物材料如真菌菌丝和孢子、水生藻类、培养细胞、昆虫胚胎及幼虫等经处理后会产生较大的变形,无法观察到生物体的原始面貌。而通过改进细菌样品固定的前处理技术,利用环境扫描电镜(ESEM)可观察到细菌样品的自然原始形态。The application of conventional high vacuum scanning electron microscopy of biological sample must be dried and treated conductively, but for both of the freeze drying or critical point drying, there exist problems that the samples have different degrees dehydration distortion. A lot of biological materials such as fungal hyphae and spores, aquatic algae, culture cell, insects, embryo and larvae processed can produce large deformation, and the original appearance of organisms can not be observed. This study improved the bacterial samples through fixed preparation technology, and the bacteria samples of natural original form can be observed by the environmental scanning electron microscope （ESEM）.

关 键 词：环境扫描电镜 生物样品 原始形态 技术改进 

分 类 号：Q248[生物学—细胞生物学]