食管癌中p53过表达、nm23低表达及其与预后的关系  

Correlation of p53 over-expression and nm23 low-expression with the Prognosis of Patients in Esophageal Squamous Cell Carcinoma

作　　者：周保平[1] 李维春[1] 周彩云[1] 

机构地区：[1]滁州市第一人民医院,安徽239000

出　　处：《安徽卫生职业技术学院学报》2013年第2期83-84,共2页Journal of Anhui Health Vocational & Technical College

摘　　要：目的:探讨nm23和p53基因在食管癌中的表达及其与食管癌临床预后关系。方法:选取即用型nm23和p53单克隆抗体对58例食管癌组织标本进行免疫组化染色标记,并进行分期对比分析。结果:58例食管癌标本中I-Ⅱ期30例,Ⅲ-Ⅳ期28例。免疫组化nm23阳性46.6%(27/58)及p53阳性58.6%(34/58)。阳性率与患者的性别、年龄及肿瘤大小无相关性。在食管癌Ⅲ-Ⅳ期中:有淋巴结转移组的nm23表达率显著低于无淋巴结转移组(P<0.05);p53的表达与食管癌组织的分化程度有相关性(P<0.05),无淋巴结转移组的表达率与有淋巴结转移组的差异无统计学意义(P>0.05)。结论:检测p53和nm23基因对食管癌中的临床分期、分化程度、预后评估具有一定的价值。Objective：To investigate expression of nm23 and p53 in human esophageal squamous cell carcinoma （ESCC）and relationship with the clinical pathologic features and prognosis of the patients.Methods ：Dilute monoelonal antibodies of nm23 and p53 as immunohistoehemieal markers with EnVision. methods in 58 specimens of ESCC tissue were established, and analyzed in TNM elassitieative. Results：The 58 eases included 3 eases of stages 1,27 eases of stages lI and 28 cases of stages 11] and IV. The immunohistoehemieal staining showed that nm23 and p53 were positive in 46.6% and 58.6% respectively. The positive rates had no correlation with the patient＇s gender, age and tumor length.In the ESCC stage Ill ,higher rate of nm23 positive staining was also found without lymph mode metastasis than that with lymph node involvement （P〈O.O5）;The expression of p53 was related to tumor differentiation and invasiveness （P 〈 0.05）; Statistical analysis did not show significant difference in expression rate of p53 without lymph node metastasis and that with lymph node involvement （P〉0.05） in ESCC stage m .Conclusion： The combined detection of nm23 and P53 gene proteins may play an important role in the progression of sguamous cell carcinoma of esophagus for cancer cell differentiation, the clinical stages and the prognosis of patients.

关 键 词：食管癌 P53 NM23 预后 

分 类 号：R735.1[医药卫生—肿瘤]