青海大通上孙家寨古代居民mtDNA遗传分析  

Genetic Analysis of People Who Lived in Shangsunjiazhai,Datong Qinghai as Revealed by Mitochondrial DNA

作　　者：张芃胤 徐智[1,2] 许渤松[1,2] 韩康信[3] 周慧[4] 金力[1,2] 谭婧泽[1,2] 

机构地区：[1]复旦大学现代人类学教育部重点实验室,上海200433 [2]上海人类学学会,上海200433 [3]中国社会科学院考古研究所,北京100710 [4]吉林大学生命科学学院,长春130012

出　　处：《人类学学报》2013年第2期204-218,共15页Acta Anthropologica Sinica

基　　金：国家自然科学基金资助项目(31071102);上海市哲学社会科学规划课题(2010BZH005)

摘　　要：利用古DNA手段对考古发掘出土的人类遗骸进行遗传分析,是揭示当地古代人群来源的重要手段。我们通过克隆测序和PCR-RFLP的方法,从来自青海大通上孙家寨的约3000-3300年前和2000年前两个不同年代的牙齿样本中,成功得到59个线粒体高变I区和编码区的SNP位点的序列信息。之后我们将所得序列与来自亚洲大陆的34个现代人群共1833个个体和2个不同年代的古代人群样本的线粒体序列分别在个体和群体水平上作比较,结果表明这两个时期人群并不是一脉相承的。Ancient DNA analysis has been used to reveal the ethnic origins of ancient human remains. In this paper, we analyzed 59 human teeth excavated from two sites near Shangsunjiazhai, Datong in Qinghai, dated between 3000/3300 and 2000 years before present. The polymorphisms in the first hypervariable region and coding regions of mitochondrial DNA were analyzed using cloning sequencing and RFLE Results were compared with 1833 mtDNA profiles from 34 contemporary populations and two ancient northwestern Chinese populations at both population and individual levels. Our results showed that people who lived in these two different periods were not the same ethnic population.

关 键 词：古DNA 线粒体DNA 卡约文化 古羌族 

分 类 号：Q987[生物学—遗传学]