以α-葡萄糖苷酶活性抑制率为指标优化山茱萸果肉的提取条件  

Optimization of extraction condition of Cornus officinalis flesh in taking inhibition rate of α-glucosidase activity as index

作　　者：李慧敏[1] 刘艳妮[1] 王小蕊[1] 康杰芳[1] 

机构地区：[1]陕西师范大学教育部药用资源与天然药物化学教育部重点实验室西北濒危药材资源开发国家工程实验室生命科学学院,陕西西安710062

出　　处：《植物资源与环境学报》2013年第1期112-114,共3页Journal of Plant Resources and Environment

基　　金：国家"十二五"科技支撑计划项目(2011BA106B06);西安市科技创新支撑计划项目[CXY1018(3)]

摘　　要：山茱萸（Comus officinalis Sieb．et Zucc．）的干燥成熟果肉是三大名贵木本药材之一。新鲜山茱萸果肉中含有单糖、多糖、有机酸、苷类、环烯醚萜类、黄酮、蒽醌、甾体和内酯等成分，临床上多用山萸肉和酒山萸肉；山茱萸是常用的降血糖药物，常出现在中医降血糖的组方中。Taking an inhibition rate of α-glucosidase activity as index, four factors including solid-liquid ratio, volume ratio of ethanol, time and temperature of ultrasonic treatment were selected to carry out single factor and orthogonal L9 （ 34 ） experiments, extraction condition of Comus officinalis Sieb. et Zucc. flesh were optimized and selected. The results show that ultrasonic treatment temperature is the most factor related to inhibition rate of ethanol extract from C. officinalis flesh to α-glucosidase. The optimization extraction condition is solid-liquid ratio 1 ： 15 （ W ： V）, 50% ethanol （ volumn ratio）, ultrasonic treatment temperature 70 ℃ and ultrasonic treatment time 30 min. And inhibition rate of flesh extract obtained under the optimization extraction condition is the highest （98.5%）. The hemi-inhibitory concentration （IC50） of extract from C. officinalis flesh to α-glucosidase is 409.30 mg·L^-1 , which is much more lower than that of the control of acarbose （890.48 mg·L^-1）.

关 键 词：山茱萸果肉 乙醇提取物 提取条件 Α-葡萄糖苷酶活性 抑制率 正交实验 

分 类 号：Q949.763.4[生物学—植物学] R284.2[医药卫生—中药学]