Alzheimer病患者外周血淋巴细胞Akt及其活化水平的改变及意义  

Change and its significance of level of Akt and its activation in peripheral blood lymphocytes in patients with Alzheimer disease

作　　者：左宝旭[1] 王珊[1] 生晓娜[1] 张国华[1] 赵辉[1] 苗江永[1] 

机构地区：[1]河北医科大学第二医院神经内科河北省神经病学重点实验室,石家庄050000

出　　处：《临床神经病学杂志》2013年第2期84-86,共3页Journal of Clinical Neurology

基　　金：河北省自然基金(C2011206110)

摘　　要：目的研究Alzheimer病(AD)患者外周血淋巴细胞Akt及其活化水平的改变及意义。方法对20例AD患者(AD组)进行简易精神状态检查(MMSE)量表评分,用免疫印迹法检测其外周血淋巴细胞的Akt及磷酸化Akt(p-Akt)水平,并与帕金森病(PD)患者(PD组)和正常对照者(正常对照组)进行比较。分析AD患者外周血淋巴细胞的Akt水平与病程和MMSE量表评分的关系。结果与正常对照组相比,AD组外周血淋巴细胞中Akt及p-Akt水平明显升高(均P<0.01);PD组p-Akt水平显著增高(P<0.05)。与PD组相比,AD组Akt水平升高明显(P<0.05)。AD患者外周血淋巴细胞中Akt水平与病程呈正相关(r=0.602,P=0.014),与MMSE量表评分呈负相关(r=-0.560,P=0.024)。结论 AD患者外周血淋巴细胞的Akt及其活化水平显著增高,并且病程越长、病情越重者增高的越明显。提示Akt有可能成为AD诊断及病情评价的外周标志物。Objective To investigate the change and its significance of level of Akt and its activation in peripheral blood lymphocytes in patients with Alzheimer disease （AD）. Methods Twenty AD patients （AD group） were taken into mini mental state examination （MMSE） tests. Their Akt and phosphated Akt （p-Akt） levels of peripheral blood lymphocytes were detected by western blotting, and then the results were compared with that of Parkinson disease （PD） patients （PD group） and normal controls （NC group）. The relationship among the level of Akt, the disease duration and MMSE score were analyzed. Results Compared with NC group, the levels of Akt and p-Akt in peripheral blood lymphocytes were significantly increased in AD group （ all P 〈 0. 01 ） ; and the level of p- Akt in peripheral blood lymphocytes in PD group was significantly increased （ P 〈 0. 05 ）. Compared with PD group, the level of Akt in peripheral blood lymphocytes in AD group was significantly increased （ P 〈 0. 05 ） . The level of Akt in peripheral blood lymphocytes in AD patients were positively correlated with the disease duration （ r = 0. 602, P = 0. 014）, and were negatively correlated with the MMSE score （ r = - 0. 560, P = 0. 024）. Conclusions The level of Akt and its activation in peripheral blood lymphocytes in AD patients are significantly increased. And the disease duration longer or conditions more severe, the increase more obvious. Which suggest that Akt may be a potential peripheral biomarker of early diagnosis and patient＇s condition assessment of AD.

关 键 词：AKT ALZHEIMER病 帕金森病 淋巴细胞 

分 类 号：R749.1[医药卫生—神经病学与精神病学]