经颅注射无水乙醇建立大鼠脑损伤模型初步探讨  

Transcranial Injection of Ethanol for Establishing Brain Injury Model in Rat

作　　者：贺继刚[1] 沈振亚[1] 滕小梅[1] 丁英龙[1] 

机构地区：[1]苏州大学附属第一医院,苏州215006

出　　处：《实验动物与比较医学》2013年第2期117-120,共4页Laboratory Animal and Comparative Medicine

基　　金：“苏州市应用基础研究计划项目”编号SYS201229;“江苏省医学重点学科”编号XK201118

摘　　要：目的探讨如何经颅注射无水乙醇建立大鼠脑损伤模型。方法10只SD大鼠，采用经颅注射无水乙醇建立大鼠脑损伤模型。结果大鼠脑损伤模型成功率为100％。10只脑损伤大鼠，均存活。10只假手术组大鼠也全部存活。实验组及假手术组术后4周体重无显著差异（376．1±11．5g vs 353．2±10．3g；P=1．534）。日饮食量也无显著差异（23．4±3．1gVS19．8±2．3g；P=0．723）。通过脑核磁共振、病理HE染色、及脑组织切片TTC染色检测可以证实建模成功。结论经颅注射无水乙醇建立大鼠脑损伤模型较传统大鼠脑损伤模型的建立操作简单、模型成功率高，结果可靠。Objective To establish a stable rat model of brain injury. Methods Ten Rats were transcranially injected with ethanol. Results The success rate of rat brain injury model is 100%, and 10 sham-operated rats survived too. After four weeks experimental group and sham group was no significant difference in body weight and daily food intake. （376.1 ± 11.5 g vs 353.2±10.3 g; P=1.534）; （23.4± 3.1 g vs 19.8 ± 2.3 g; P=0.723）. By brain magnetic resonance imaging, pathology HE staining, and brain tissue slices TTC staining can confirm modeling success. Conclusion Transcranial injection of ethanol to establish rat brain injury model is simple. Success rate is higher than traditional methods. A solid foundation is established for further studies on brain injury.

关 键 词：脑损伤 模型 大鼠 

分 类 号：R-332[医药卫生] R651.15