乳腺浸润性导管癌中HIF-1α与Twist1蛋白的表达与分析  

Expression and analysis of HIF - 1 α and Twistl Proteins in invasive ductai carcinoma of breast

作　　者：吴狄[1] 李荣[1] 罗荣城[1] 

机构地区：[1]南方医科大学南方医院肿瘤中心,广州510515

出　　处：《实用肿瘤学杂志》2013年第2期144-148,共5页Practical Oncology Journal

摘　　要：目的探讨乳腺浸润性导管癌中HIF-1α、Twist2蛋白表达及其临床意义。方法采用免疫组织化学SP法检测41例乳腺浸润性导管癌患者的癌组织与配对癌旁正常乳腺组织中HIF-1α与Twistl蛋白的表达情况。结果癌组织中HIF—1α与Twistl的阳性表达率明显高于癌旁正常乳腺组织（HIF-1α：78．0％vs19．5％，P〈0．001；Twistl：63．4％vs．12．2％，P〈0．001）。在癌组织中，HIF-1α与Twistl的表达分别与TNM分期呈正相关（HIF-1α：r=0．322，P=0．04；Twist1：r=0．430，P=0．005），HIF-1α与Twist1蛋白的表达呈正相关（r=0．331，P=0．034）。结论乳腺浸润性导管癌中HIF-1α．与Twist1蛋白呈高表达，且两者具有相关性，两者在乳腺浸润性导管癌的发生发展过程中可能起着重要的作用。Objective The study investigated the expressions and significance of hypoxia - inducible factor- 1α（ HIF- 1α ）and Twist l in invasive ductal carcinoma of breast （IDC）. Methods The expressions of HIF- lot and Twistl proteins were detected by immunohistochemistry streptavidin perotridase （SP）method in breast carcinoma tissue and paired para - carcinoma tissues of 41 patients with IDC. Results The positive ex- pression rates of HIF - 1α and Twistl proteins in breast carcinoma tissues were significantly higher than in paired para - carcinoma tissues （ HIF - 1α ：78.0% vs. 19.5 %, P 〈 0.001 ; Twist1：63. 4% vs. 12.2%, P 〈 0. 001 ）. In breast carcinoma tissues, the expressions of HIF - 1α and Twistl in breast carcinoma tissues were both positively correlated with the TNM stage（HIF - lot：r =0. 322,P =0.04;Twistl r =0.43 ,P =0. 005） ,and the expression of HIF - lot was positively correlated with the expression of Twistl （ （ r =0.336 ,P =0.019） ） in breast carcinoma tissues. Conclusion The overexpressions of HIF - lot and Twistl were found in IDC and their expressions were positively correlated. They might play an important role in the development of IDC.

关 键 词：乳腺癌 免疫组织化学 乏氧诱导因子-1 TWIST 

分 类 号：R737.9[医药卫生—肿瘤]