一株产油酵母菌的微波诱变选育  

Breeding of a strain of oleaginous yeast via the microwave mutation

作　　者：马勇[1] 陈秀莉[1] 张应龙[2] 门中华[1] 

机构地区：[1]包头师范学院生物科学与技术学院,内蒙古包头014030 [2]内蒙古科技大学生物工程与技术研究所,内蒙古包头014010

出　　处：《广东农业科学》2013年第6期118-119,123,共3页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31160254);校内青年科学研究基金(BSYKJ2011-17)

摘　　要：以产油酵母菌Y1为试验菌株,通过对出发菌株Y1的最佳微波诱变时间确定、诱变后菌株初筛和复筛后得到一株突变菌株Y13,测得Y13的油脂产量4.39 g/L、油脂含量22.09%,比出发菌株Y1油脂产量2.11 g/L、含油量9.6%明显提高。因此,菌株Y13可作为后续摇瓶发酵产脂研究的试验菌株。The mutant strain Y13 was isolated by the time of optimum microwave mutagenesis, mutagenic strains of screening and re-screening within oleaginous yeast Y1. The results showed that Y13 was significantly improved in oil production and content, the oil production reached 4.39 g/L from 2.11 g/L and the content reached 22.09% from 9.6%, respectively. It is feasible to employ Y13 as original strains using shake-flask fermentation for lipid-producing.

关 键 词：产油酵母 微波诱变 诱变选育 油脂产量 

分 类 号：TS261.43[轻工技术与工程—发酵工程]