乏氧诱导因子-1α在人牙周膜细胞的表达  

Effects of Hypoxia on the Expression of HIF-1α by Human Periodontal Ligament Cells in Vitro

作　　者：唐开亮[1] 于西佼[1] 杜毅[1] 

机构地区：[1]济南市口腔医院,山东济南250012

出　　处：《中国医学创新》2013年第13期6-7,共2页Medical Innovation of China

摘　　要：目的:本研究在缺氧的环境下培养人牙周膜细胞,模拟类似牙周组织局部缺血的病理模型,探讨体外牙周膜细胞在缺氧环境中HIF-1α的表达情况。方法:常规培养人牙周膜细胞,20%和1%的氧浓度下培养牙周膜细胞24h,利用RT-PCR及western blot技术检测HIF-1αmRNA及蛋白的表达情况。结果:在常氧及缺氧环境下,都可检测到HIF-1αmRNA的表达,且两者的表达量差异无统计学意义,但在缺氧环境中牙周膜细胞的HIF-1α蛋白呈强阳性表达,与对照组比较,差异有统计学意义。结论:缺氧使牙周膜细胞HIF-1α蛋白表达增加,提示牙周炎和正畸导致的缺氧微环境对人牙周膜细胞的活性与功能有一定影响。Objective： To investigate the effects of hypoxia on expression of HIF-1α in human periodontal ligament cells.Method： Human periodontal ligament cells （ hPDLCs ） were cultured under 20% and 1% 02 for 24h. RT-PCR and westblot metheod was used to localize the distribution of HIF-1α and VEGF in hPDLCs. Result： There were no significant differences between the hypoxie group and the normoxic group about the expression of HIF-1α mRNA in hPDLCs. But the expression of HIF-1α protein in hypoxia groups were significantly higher than in the control groups.Conclusion： It is suggested that there is a significant effect of hypoxia on PDL cells on the expression of HIF-1α.

关 键 词：牙周膜细胞 缺氧 乏氧诱导因子 

分 类 号：R781.4[医药卫生—口腔医学]