黑木耳野生及栽培菌株对重金属Cd的耐受与富集  

Tolerance and Bioaccumulation Characteristics of Wild Strains and Cultivated Strains of Auricularia auricula-judae to Heavy Metal Cd

作　　者：柴红梅[1] 汪禄祥[2] 和丽忠[2] 赵静[1] 黎其万[2] 赵永昌[1] 

机构地区：[1]云南省农业科学院生物技术与种质资源研究所,云南昆明650221 [2]云南省农业科学院质量标准与检测技术研究所,云南昆明650221

出　　处：《西南农业学报》2013年第2期681-685,共5页Southwest China Journal of Agricultural Sciences

基　　金：国家科技支撑项目"重要农产品主要争议性重金属限量指标的再评价"(2009BADA7B06)

摘　　要：选择黑木耳(Auricularia auricula-judae)的2株野生菌株(YAASM1399、YAASM1402)和2株栽培菌株(YAASM2691、YAASM2692),在实验室条件下进行平板和摇瓶培养,测定4株菌株在不同Cd浓度下对Cd的耐受及富集能力。结果表明,2株野生菌株对Cd的耐受力强于2株栽培菌株,低浓度(<2 mg/L)Cd对菌株YAASM1399的生长有促进作用;比较4株菌株菌丝体中Cd浓度,发现Cd的富集性能菌株YAASM2691相对最强,YAASM1399最弱;低Cd浓度时菌株YAASM2692对Cd的去除率最高,但对Cd的耐受力较差。综合考虑耐受力、Cd富集倍数等因素,认为4株菌株中YAASM1402较适合为Cd污染生态修复菌株。Two wild strains and two cultivated strains of Auricularia auricula-judae were selected to investigate the growth tolerance and bioac-cumulation characteristics under different concentrations of Cd in terms of colony diameter, dry weight of mycelia and Cd content in mycelia. The results showed that the Cd tolerance of the two wild strains were better than that of the two cultivated strains. Low concentration Cd（ 〈 2 mg/L ） promoted the growth and dry weight of mycelia of wild strain YAASM1399 while had little effect on the other wild strain YAASM1402, but the growth rate and dry weight of mycelia of two cultivated slrains were significantly inhibited. The Cd content in mycelia of four strains increased with the increase of Cd concentration in liquid media and the abilities of bioaccumulation to Cd of strain YAASM2691 was relatively stronger than the other strains. Although the removal rate to Cd was higher than the other three strains at low Cd concentration（ 〈2 mg/L ） ,YAASM2692 could not grow at high Cd concentration. Considering the growth tolerance and bioaccumulation a-bilities to Cd, YAASM1402 was more suitable than the other three strains to remove Cd from Cd pollution ecological environment.

关 键 词：野生菌株 栽培菌株 Cd耐受 菌丝生长 黑木耳 

分 类 号：S646[农业科学—蔬菜学]