饲用芽孢杆菌混菌发酵产中性蛋白酶的工艺优化  

OPTIMIZATION OF PRODUCTION PROCESS OF NEUTRAL PROTEASE FROM BACILLUS STRAINS BY MIXTURE-CULTURE FERMENTATION

作　　者：潘进权[1] 曾伟文[1] 邱玉娜[1] 李家冬[1] 

机构地区：[1]湛江师范学院生命科学与技术学院,广东湛江524048

出　　处：《河南工业大学学报（自然科学版）》2013年第2期93-99,共7页Journal of Henan University of Technology：Natural Science Edition

基　　金：广东省自然科学基金项目(9452404801001943);湛江市科技攻关计划项目(2011C3103010)

摘　　要：为提高饲用芽孢杆菌中性蛋白酶的发酵单位,考察了多种芽孢杆菌混菌发酵产蛋白酶的协同作用效果,并在单因素试验的基础上,先后采用部分析因设计、爬坡设计及中心组合设计的试验方法对菌株配伍方式及发酵培养基组成进行了优化.通过优化构建了合适的混菌发酵体系,即芽孢杆菌A1、B1、B3菌株的混合比例为2∶3∶3;最佳发酵培养基组成(g/L)为:麦芽糖58.5、酵母膏28.6、麸皮42.5、吐温80 3.0、K2HPO43.0、CaCO33.0.在优化条件下,芽孢杆菌混菌发酵产中性蛋白酶活力单位可达到6 728 U/mL,较优化前芽孢杆菌A1的发酵活力单位提高了175.6%.To increase the fermentation titer unit of neutral protease from Bacillus strains, we studied the synergistic effect of multiple kinds of Bacillus strains for preparing neutral protease by mixture-culture fermentation, and optimized the combination manner of strains and the composition of the fermentation medium by single-factor experiments, fractional factorial design, steepest ascent design and central composite design to construct a proper mixture-culture fermentation system, which contained Bacillus strain A1, B1 and B3 at a ratio of 2 ：3 ： 3. The results showed that the optimum fermentation medium contained maltose 58.5 g/L,yeast extract 28.6 g/L,wheat bran 42.5 g/L, Tween80 3.0 g/L,K2HPO4 3.0 g/L,and CaCO3 3.0 g/L. Under these optimized conditions, the active unit of neutral protease produced from Bacillus strains by mixture-culture fermentation reached 6 728 U/mL, which was increased by 175.6 % in comparison to that of Bacillus strain A1.

关 键 词：芽孢杆菌 混菌发酵 中性蛋白酶 中心组合设计 

分 类 号：Q939.97[生物学—微生物学]