两种药用植物总黄酮体外抗氧化活性比较  

Comparation of Antioxidant Activity Between Total Flavonoids Isolated from Two Different Plants in vitro

作　　者：黄秋洁[1] 叶勇[2,3] 欧贤红[4] 刘华钢[2] 宋云飞 

机构地区：[1]广西中医药大学药学院,南宁530001 [2]广西医科大学药学院,南宁530021 [3]桂林莱茵生物科技股份有限公司博士后工作站,广西桂林541100 [4]桂林医学院药学院,541004

出　　处：《医药导报》2013年第5期576-578,共3页Herald of Medicine

基　　金：广西教育厅基金资助项目(201010LX340)

摘　　要：目的研究藤茶总黄酮和铁包金总黄酮的体外抗氧化活性。方法建立体外二苯代苦味酰基自由基(DPPH·)、氧自由基(O-2·)和羟自由基(·OH)发生体系,检测藤茶总黄酮和铁包金总黄酮对DPPH·、O-2·和·OH的清除作用。结果藤茶总黄酮对DPPH·、O-2·、·OH的清除作用比铁包金总黄酮和维生素C强,其三种体系的半数抑制浓度(IC50)分别为(3.32±0.18),(227.41±184.45),(5.22±3.75)mg·L-1。铁包金总黄酮对DPPH·、O-2·、·OH的清除作用弱于维生素C,其DPPH.体系的IC50为(36.41±9.68)mg·L-1。结论藤茶总黄酮具有抗氧化活性,但铁包金总黄酮不能单独作抗氧化剂。Objective Antioxidation of total flavonoids from Ampelopsis grossedntata and Berchemia lineata （ L. ） DC was studied in vitro. Methods Scavenging effects of total flavonoids from Ampelopsis grossedntata and Berchemia lineata（L. ） DC on DPPH free radical, hydroxyl free radical, and superoxide free radical were determined by ELISA. Results The activity of total flavonoids from Ampelopsis grossedntata was the strongest,with the IC50 of three systems being （3.32±0.18）, （227.41±184.45 ）, （5.22±3.75） mg·L^-1, respectively, while the activity of total flavonoids from Berchemia lineata（ L. ） DC was the lowest, with the IC50 of DPPH·system being （36.41±9.68） mg·L^-1. Conclusion The total flavonoids from Ampelopsis grossedntata have strong antioxidant activity , but the total flavonoids from Berchemia lineata （L.） DC could not be employed asantioxidant alone.

关 键 词：藤茶 铁包金 总黄酮 抗氧化 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]