北京地区男男性接触人类免疫缺陷病毒感染者人类白细胞抗原-Ⅰ类分子多态性对病毒载量的影响  

The influence of human leucocyte antigen-I polymorphisms on plasma viral load in human immunodeficiency virus infected male homosexual population in Beijing

作　　者：张欣[1] 王熠[1] 王爽[1] 李伟华[1] 胡文静[1] 赵丹彤[1] 闫惠平[1] 

机构地区：[1]首都医科大学附属北京佑安医院感染与免疫研究中心,100069

出　　处：《中华传染病杂志》2013年第3期173-178,共6页Chinese Journal of Infectious Diseases

基　　金：北京市科学技术委员会资助重大项目（D0905070359094,D0906003040391）;北京市初级卫生保健基金会佑安肝病艾滋病基金（BJYAH一201I-024）

摘　　要：目的分析北京地区男男性接触人群HIV感染者人类白细胞抗原（HLA）-I的多态性及其对病毒载量的影响。方法用序列特异性引物一聚合酶链反应（SSP—PCR）对157例慢性HIV感染者的HLA—A、HLA-B、HLA-C等位基因分型，同时检测HIV载量。正态分布的计量资料采用单因素或多因素方差分析，非正态分布的计量资料用Mann-WhitneyU检验。结果157例HIV感染者中，HLA-B携带Bw4表位簇个数与低病毒载量有关（F=3．01，P=0．045），HLA-B携带Bw4／4纯合子的感染者HIV载量为（4．19±0．76）lgIU／mL，Bw6／6纯合子的感染者为（4．63±0．74）lgIU／mL（t=2．27，P=0．010）。HLA—A、HLA—B同时携带3个Bw4的感染者HIV载量为（3．92±0．97）lgIU／mL，显著低于携带1个Bw4的感染者HIV载量（4．54-4-0．88）lgIU／mL和不携带Bw4者HIV载量（4．60±0．72）lgIu／mL（t=2．11，P=0．039；t=2．53，P=0．015）。HLA—I类分子（HLA—A、HLA—B、HLA—c）均携带杂合子的感染者，其病毒载量与任一座位携带纯合子的感染者病毒载量比较，差异无统计学意义。HLA_I类分子均携带杂合子且HLA—B携带Bw4／4纯合子的感染者其HIV载量中位数为4．。glgIU／mL，低于HLA-B携带Bw6／6纯合子感染者的4．551gIU／mL（U=210．50，P=0．041）。携带A30／B13／C06单体型或A33／B58／C03单体型的感染者其HIV载量与不携带A30／B13／C06单体型或A33／B58／C03单体型感染者比较，差异无统计学意义（t=0．40，P=0．69；t=0．68，P=0．49）。结论HIV感染者HLA—B携带Bw4／4纯合子与低病毒载量有关，且HLA-I类分子携带杂合子的个体可受HLA—B携带Bw4／4纯合子影响，这些HIV感染者病毒载量更低。Objective To analyze the influence of the polymorphisms of human leucocyte antigen （HLA）-I molecule and the effects on plasma viral load of human immunodeficiency virus （HIV） infected male homosexual population in Beijing. Methods The HLA-A, HLA-B, HLA-C allele were typed by sequence specific primer-polymerase chain reaction （SSP-PCR）, and viral load was detected in 157 chronic HIV infected persons. Normally distributed measurement data were analyzedby one-way or multi-way analysis of variance, while data of abnormal distributions were analyzed by Mann-Whitney U test. Results Among 157 chronic HIV infected persons, the number of Bw4 motifs on HLA-B loci was associated with a lower level of viral load （F=3.01~ P=0. 045）. In these HIV infected persons, the viral load in HLA-B carrying Bw4/4 homozygote was （4.19~0.76） lg IU/mL, in HLA-B carrying Bw6/6 homozygote was （4.63d：0.74） lg IU/mL （t=2.27, P=0. 010）. The viral load of those who carried three, one or none Bw4 motifs on HLA-A and HLA-B loci were （3.92＋_ 0.97）, （4.54~0. 88） and （4. 60~0.72） lg IU/mL, respectively （three vs none： t=2. 53, P= 0. 015 ~ three vs one~ t=2.11, P=0. 039）. HIV infected persons who carried homozygote on any loci of HLA-A, HLA-B, HLA-C had comparable levels of plasma viral load to those who carried heterozygote on HLA-A, HLA-B, HLA-C loci. Among the persons who carried heterozygote on HLA-A, HLA-B, HLA-C loci, Bw4/4 homozygote on HLA-B had lower levels of viral load than Bw6/6 homozygote on HLA-B （median： 4.09 lg IU/mL vs 4.55 lg IU/mL, U=210.50, P=0. 041）. HIV infected persons who carried A30/B13/C06 or A33/B58/C03 haplotype had comparable levels of plasma viral load to those without A30/B13/C06 or A33/B58/C03 haplotype （t= 0.40, P=0.69; t= 0.68, P= 0.49, respectively）. Conclusions Bw4/4 homozygote on HLA-B loci is associated with lower HIV viral load. Furthermore, the plasma viral load of HIV infected persons carrying heterozygote on HLA-A, HLA-B, HLA-C loci could be influenced

关 键 词：HIV感染 HLA-B抗原 纯合子 病毒载量 单体性 

分 类 号：R51[医药卫生—内科学]