血清8-羟基脱氧鸟嘌呤水平与Graves眼病关系的临床研究  

The association between 8-hydroxy-deoxyguanosine and Graves＇ ophthalmopathy

作　　者：仇春健[2] 叶小珍[1] 王扬天[1] 张敏娟[1] 王坚[1] 

机构地区：[1]南京大学医学院临床学院,南京军区南京总医院内分泌科,210002 [2]解放军第81医院内分泌科

出　　处：《中华内分泌代谢杂志》2013年第4期322-323,共2页Chinese Journal of Endocrinology and Metabolism

基　　金：国家自然科学基金资助项目（81070626）

摘　　要：探讨血清8-羟基脱氧鸟嘌呤（8-hydroxy-deoxyguanosine,8-OHdG）水平在Graves眼病发病机制中的作用及与Graves眼病活动性的关系。用酶联免疫吸附法（ELISA）检测48例Graves眼病患者（GO组）和30例单纯Graves病而无眼征的患者（GD组）的血清8-OHdG水平，同时检测30名健康体检者作为对照组。与对照组相比，GO组和GD组的血清8-OHdG水平明显增高［（2.98 ± 1.33）,（2.07 ± 1.30）对（0.72 ± 0.93）ng/ml, P〈0.05］；且GO组的血清8-OHdG水平明显高于GD组（P〈0.05）；Pearson相关性分析显示血清8-OHdG水平与临床疾病活动分数呈显著正相关（r=0.54,P〈0.01）。Graves眼病患者外周血8-OHdG水平明显增高，提示氧化应激与Graves眼病的发病机制有关，血清8-OHdG水平可作为评价Graves眼病疾病活动度的良好指标。To investigate the role played by 8-hydroxy-deoxyguanosine（8-OHdG） in patients with Graves′ ophthalmopathy（GO）, and the association between 8-OHdG and the disease activity of GO. Serum levels of 8-OHdG were tested in 48 cases with GO and 30 cases with Graves′ disease（GD） without ophthalmopathy. 30 healthy subjects participated in this study as controls. 8-OHdG was detected by enzyme-linked immunosorbent assay（ELISA）. Compared with the controls, the mean level of 8-OHdG in GO patients and GD patients was increased significantly[（2.98 ± 1.33）,（2.07 ± 1.30） vs（0.72 ± 0.93）ng/ml, P〈0.05]. Mean 8-OHdG level in GO patients was markedly higher when compared to GD patients（P〈0.05）. Pearson regression analysis showed that elevated levels of 8-OHdG were significantly correlated with the Clinical Activity Score of GO（r=0.54,P〈0.01）. The serum level of 8-OHdG in GO patients was raised obviously, it implies that oxidative stress plays an important role in the pathogenesis of GO, and the serum level of 8-OHdG might act as an activity index of GO.

关 键 词：氧化应激 血清8-羟基脱氧鸟嘌呤水平 格雷夫斯眼病 

分 类 号：R77[医药卫生—眼科] R5[医药卫生—临床医学]