CD151基因转移对大鼠缺血后肢Rac1和Cdc42信号通路的影响  

Effect of CD151 gene transfer on Rac1 and Cdc42 in rat ischemia hind-limb

作　　者：刘伟峰[1] 于晓晖[2] 刘正湘[3] 左后娟[3] 

机构地区：[1]淄博市中心医院心内科,山东淄博255036 [2]淄博市中心医院眼科 [3]华中科技大学同济医学院附属同济医院心内科

出　　处：《中国老年学杂志》2013年第9期2054-2056,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(No.81000047)

摘　　要：目的观察CD151在大鼠后肢缺血模型中的表达以及对Rac1和Cdc42信号通路的影响,进一步研究CD151促血管新生的分子机制。方法将实验大鼠随机分为4组,分别给予生理盐水,rAAV-GFP和rAAV-CD151分点注射至大鼠左后肢肌肉组织内,基因转染1 w后行股动脉切除术建立大鼠后肢缺血模型,假手术组不切除股动脉。6 w后用Western印迹方法检测各组大鼠缺血肢体局部CD151以及Rac1和Cdc42的表达。结果术后5 w,Western印迹检测CD151组的CD151蛋白表达水平明显高于其余各组(P<0.05),同时CD151组p-Rac1/Cdc42蛋白表达水平明显高于其余各组(P<0.05)。结论 CD151蛋白表达可以激活Rac1和Cdc42信号通路,促进缺血组织的血管新生。Objective To investigate the effects of CD151 mutant gene delivery on Cdc42 and Racl in the model of rat hind-limb ischemia. Methods Rats were randomly divided into 4 groups. Rats in 3 groups were injected with saline, rAAV-GFP, or rAAV-CDISI respectively （ n = 6/group）. After 1 week of transfection,the femoral arteries of the rats were excised to establish the model of left hind-limb ischemia. Sham-operated animals underwent the same surgical prncedure without femoral artery resection. The expression of CD151 and phos- phor-Racl/Cdc42 in the ischemic tissue was detected by Western blot. Results The expression of CD151 protein was increased significantly in rAAV-CD151 groups compared with other groups （P 〈 0. 01 ）. The expression of phosphor-Racl/Cdc42 protein were increased significantly in rAAV-CD151 groups compared with other groups （P 〈0. 01 ）. Conclusions CD151 gene transfer can activate Racl and Cdc42 in rat ischemia hind-limb.

关 键 词：CD151基因 缺血模型 血管新生 RAC1 CDC42 

分 类 号：R364.1[医药卫生—病理学]