肺肉瘤样癌中血管生成拟态的形成及其与上皮间充质转化的关系  

Relationship between vasculogenic mimicry and epithelial-to-mesenchymal transition in pulmonary sarcomatoid carcinoma

作　　者：郑旭[1] 孙保存[1,2] 赵秀兰[1] 王进京[1] 董学易[1] 古强[1] 孙冉[1] 

机构地区：[1]天津医科大学病理教研室,天津市300070 [2]天津医科大学附属肿瘤医院病理科

出　　处：《中国肿瘤临床》2013年第8期431-435,共5页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金重点项目(编号:81230050);国家自然科学基金面上项目(编号:81172046;81173091)资助~~

摘　　要：目的:研究肺肉瘤样癌(pulmonary sarcomatoid carcinoma,PSC)中血管生成拟态(vasculogenic mimicry,VM)的临床意义,阐述上皮间充质转化(epithelial-to-mesenchymal transition,EMT)提高PSC的恶性度及促进VM形成的相关机制。方法:对肺肉瘤样癌组织切片22例进行HE、CD31和PAS双重染色,Twist、Vimentin、E-cadherin、VEGF免疫组织化学染色,分析VM相关的临床意义及EMT促进VM形成的相关机制。结果:22例中4例(18.2%)存在VM,Kaplan-Meier生存分析提示有无VM肿瘤患者比较生存时间差异有统计学意义(P<0.05)。VM阳性患者Twist的阳性率较阴性患者高,且差异有统计学意义(P<0.05)。Twist1的表达与VEGF、E-cadherin、Vimentin的表达存在明显相关性。结论:VM是患者预后的不利因素,肺肉瘤样癌中发生了EMT,转录因子Twist1可能通过下调E-cadherin,上调Vimentin诱导EMT,并可能通过上调VEGF的表达促进VM的形成,从而促进肿瘤的侵袭和转移。Objective： The expression and clinical significance of vasculogenic mimicry （VM） were investigated. The related mechanisms of epithelial-to-mesenchymal transition （EMT） in VM formation and worsening of puhnonary sarcomatoid carcinoma （PSC） were examined. Methods： Hematoxylin and eosin staining, CD31/periodic acid-Schiff double staining, and immunohistochemi- cal staining were performed to determine the expression of VM, Twist l, vascular endothelial growth factor （VEGF）, E-cadherin, and vi- mentin in the tissues of 22 PSC patients. The clinical significance of VM and the mechanism of EMT in VM formation were explored. Results： VM was found in four （18.2%） of the 22 PSC patients. Kaplan Meier survival analysis revealed that the patients with VM had a shorter survival period than those without the VM expression, with statistically significant differences found between the two groups. Comparative results of the groups with and without VM expression showed a higher positive rate of Twistl expression in the former group （P〈0.05）. Significant correlations were observed between the Twistl expression and the VEGF, E-cadherin, and vimentin expression. Conclusion： VM unfavorably influences the prognosis of PSC patients. Twistl can upregulate Vimentin and downregulate E-cadherin by inducing EMT. Additionally, it may upregulate VEGF to promote VM formation, which increases tumor invasions and metastases.

关 键 词：血管生成拟态 上皮间充质转化 肺肉瘤样癌 免疫组织化学 

分 类 号：R734.2[医药卫生—肿瘤]