肿瘤易感基因101表达对胃癌细胞侵袭和转移的影响  

Changing of TSGIO1 expression on the invasion and metastasis of gastric cancer cells

作　　者：张敏[1] 王琦[1] 任晓静[1] 杨扬[1] 孙建莉[1] 何冬梅[1] 申慧琴[1] 

机构地区：[1]山西医科大学第二医院消化科,太原030001

出　　处：《中华医学杂志》2013年第16期1219-1223,共5页National Medical Journal of China

基　　金：国家自然科学基金（81071972）

摘　　要：目的探讨肿瘤易感基因101（TSG101）在胃癌细胞SGC7901侵袭和转移中的作用。方法将TSG101真核表达质粒及空质粒转染胃癌细胞SGC7901，建立稳定表达TSG101的细胞株，反转录一PCR与蛋白质印迹检测TSG101在各组细胞中的表达，设立TSG101真核表达组、质粒组和空白对照组。通过侵袭、迁移、黏附及损伤刮擦实验检测TSG101表达对胃癌细胞侵袭和转移能力的影响。统计学采用单因素方差分析，组间比较用SNK检验。结果TSG101真核表达组TSG101mRNA和蛋白表达水平均高于质粒组和空白对照组（0．85±0．09比0．55±0．07、0．45±0．07，29．4±1．2比17．0-．t-O．4、15．9±0．4，均P〈0．05）；TSG101真核表达组细胞穿过基质胶、层黏连蛋白和Ⅳ型胶原层的细胞数（84±14、128±10、62±7）均高于质粒组（554-9、77±10、31±6）与空白对照组（48±8、76±9、24±5，均P〈0．01）；TSG101真核表达组细胞黏附于铺制基质胶、层黏连蛋白和Ⅳ型胶原的细胞数（0．97±0．04、1．34±0．04、0．90±0．01）均高于质粒组（0．53±0．03、0．75±0．05、0．42±0．02）与空白对照组（0．60±0．03、0．72±0．03、0．40±0．01，均P〈0．01）；TSG101真核表达组细胞迁移至膜下表面的数量高于质粒组和空白对照组（87±13比54±8、48±7，均P〈0．01）；培养24、48hTSG101真核表达组细胞融合速度明显快于质粒组及空白对照组。结论稳定转染TSG101真核表达质粒后SGC-7901细胞表达TSG101明显增高，侵袭转移能力也明显增强。Objective To explore the functions of tumor susceptibility gene 101 （ TSG101 ） in the invasion and metastasis of gastric cancer cells by cell culture. Methods The TSG101 eukaryotic expression and empty plasmids were transfected into gastric cancer cell line SGC7901. After screening with G418,single cell clone was selected and cultured. The expression of TSG101 was detected by reverse transcription （RT） - PCR and Western blotting. Cells were divided into TSG101 eukaryotic expression plasmid and blank control groups. Then the relationship was examined between TSG101 expression and tumor invasion and metastasis through the invasion, mobile, adhesion and damage scart experiment. Results The expression levels of TSG101 in mRNA and protein in the TSG101 eukaryotic expression group were significantly higher than those of the plasmid and blank control groups （ 0. 85 ± 0. 09 vs 0. 55 ± 0. 07, 0.45 ± 0. 07 and 29.4 ~ 1.2 vs 17. 0 -± 0. 4, 15.9 ± 0. 4, all P 〈 0. 05 ）. The cell number of TSG101 eukaryotic expression group through Matrigel, laminin, typeIV collagen protein （ 84 ± 14, 128 ± 10, 62 ± 7 ） were significantly higher than those of the plasmid group（55 ±9, 77± 10, 31 ±6） and blank control group（48 ±8, 76 ±9, 24 ± 5,all P 〈 0. 01 ）. The number of cells adhereent to Matrigel, laminin, type 1V collagen protein of the TSG101 eukaryotic expression group（0. 97 ± 0.04, 1.34 ± 0. 04, 0. 90±0. 01 ） were obviously higher than thoses of the plasmid group （ 0. 53 ± 0. 03, 0. 75 ± 0. 05, 0.42 ± 0. 02 ） and blank control group （ 0. 60 ± 0.03,0. 72 ± 0. 03,0. 40 ± 0. 01, all P 〈 0. 01 ）. The number of TSG101 eukaryotic expression group cell migrating to membrane lower surface was obviously higher than that of the plasmid group and blank control group（ 87 ± 13 vs 54 ± 8, 48 ± 7, all P 〈 0. 01 ）. The fusion speed of the TSG101 eukaryotic expression group was faster than that of plasmid and blank control groups after cultivating for 24 and 48 h. Conclusions TS

关 键 词：胃肿瘤 转染 肿瘤侵润 TSG101 

分 类 号：R735.2[医药卫生—肿瘤]