健脾活血方预防急性醉酒的实验研究  

Experiment research on Jianpihuoxue Decoction in prevention of acute alcoholism

作　　者：徐琳[1] 冯琴[1] 彭景华[1] 傅琪琳[1] 黄甫[1] 赵瑜[1] 陈亮[1] 王文静[1] 孟胜喜[1] 胡义扬 

机构地区：[1]上海中医药大学附属曙光医院肝病研究所,上海201203 [2]上海市高校中医内科学E-研究院,上海201203 [3]上海市中医临床重点实验室,上海201203

出　　处：《中华中医药杂志》2013年第5期1313-1316,I0005,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.30801536;No.30371818);上海市高校创新团队(第一期);国家中医药管理局中医肝胆病重点学科项目(No.2010sh)~~

摘　　要：目的:研究健脾活血方对大鼠急性醉酒的预防作用。方法:将SD大鼠28只随机分正常组(8只)、模型组(10只)和健脾活血方组(10只)。各组预先给饮用水或药4d,2次/d,每次灌胃剂量10mL/kg体质量(生药量9g/mL)。末次给药1h后,模型组和健脾活血方组大鼠予以56%乙醇15mL/kg体质量灌胃复制大鼠酒精中毒模型。观察大鼠灌酒后翻正反射消失时间与昏睡时间。24h后留取标本,检测血清门冬氨酸氨基转移酶(AST)活性,肝及胃组织HE染色和乙醇代谢酶指标。结果:健脾活血方组大鼠灌酒后翻正反射消失时间明显延长(P<0.01),昏睡时间缩短(P<0.05);HE染色见胃黏膜上皮细胞脱落减少,肝细胞内脂肪空泡形成减少;血清AST活性显著降低(P<0.01);肝组织乙醇脱氢酶(ADH)活性显著升高(P<0.01)。结论:健脾活血方对大鼠急性醉酒有预防作用,其机制可能与减缓乙醇在体内的吸收,提高肝脏ADH活性有关。Objective： To study the preventive effect of Jianpihuoxue Decoction on acute alcoholism rats. Methods： Twenty-eight Sprague Dawley rats were allocated to normal group （8 rats）, acute alcoholism model group （10 rats） and Jianpihuoxue Decoction group （10 rats）. Each group was given the Jianpihuoxue Decoction or water with the average does of 10mL/kg （equivalent to 9g/kg crude drug） twice a day for four days. After one hour of the last dose, the acute alcoholism model group and Jianpihuoxue Decoction group were given 56% ethanol 15mL/kg to reproduce the model of acute alcoholism. The righting reflex was observed. The specimen was taken after 24h. HE staining was performed to observe pathological changes in liver and stomach samples. Activities of serum AST, ADH and hepatic ADH were tested. Results： Jianpihuoxue Decoction prolongs the absence of the righting reflex （P〈0.01） and shortens the sleeping time （P〈0.05）; alleviates microvesicular steatosis in the hepatocytes and desquamation of gastric epithelial cell by HE staining; reduces activities of serum AST （P〈0.01）; increases hepatic ADH （P〈0.01）. Conclusion： Jianpihuoxue Decoction has the preventive effect on acute alcoholism rats by slowing ethanol absorption and promoting the activities of hepatic ADH.

关 键 词：健脾活血方 急性醉酒 乙醇代谢酶 

分 类 号：R285.5[医药卫生—中药学]