愤怒、郁怒反应模型大鼠不同脑区5-羟色胺含量分析  

Analysis of 5-HT content in different brain regions of the anger-in and anger-out model rats

作　　者：魏盛[1] 宗绍波[1] 乔明琦[1] 

机构地区：[1]山东中医药大学,济南250355

出　　处：《中华中医药杂志》2013年第5期1423-1426,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.81202617;No.30973688;No.81173163)~~

摘　　要：目的:在微观水平上评价及区分愤怒和郁怒反应模型大鼠的指标,并进一步探讨愤怒、郁怒发生的微观机制。方法:采用社会隔离结合居住入侵方法制备愤怒、郁怒大鼠模型,分别为正常对照组、愤怒模型组、郁怒模型组;并运用HPLC检测各组大鼠不同脑区5-HT的含量变化。结果:与正常对照组相比,愤怒模型组大鼠下丘脑5-HT含量显著降低(P<0.001),郁怒模型组大鼠额叶皮质5-HT含量显著上升(P<0.05),而下丘脑5-HT含量则显著下降(P<0.001);与郁怒模型组相比,愤怒模型组大鼠下丘脑5-HT含量显著上升(P<0.05)。结论:大鼠愤怒、郁怒情绪反应与下丘脑5-HT含量显著下降密切相关,该结论为进一步探讨怒情绪发病机制提供理论奠基和方向。Objective：To explore the microscopic molecular biological indicators evaluated and distinguished between anger-in and anger-out reaction rat models, and to further explore the anger-in and anger-out occurrence of the microscopic mechanism. Methods：The anger model rats were prepared by a social isolation plus resident-intruder stress, respectively, into a normal group, an anger-in emotion rat model group and an anger-out emotion rat model group. 5-HT content in different brain regions of rats were measured by high performance liquid chromatography （HPLC）. Results：Compared with the normal group, the content of 5-HT in anger-out/in model rats hypothalamus significantly decreased （P〈0.001）, while increased in prefrontal cortex significantly （P〈0.05）, 5-HT contents of anger-out model group in hypothalamus were obviously higher than that of anger-in group （P〈0.05）. Conclusion： Anger-out and anger-in emotional reactions in model rats are related with 5-HT content significant decreasing in hypothalamus, which provids theoretical basis and direction for the further study of anger emotion pathogenesis.

关 键 词：大鼠模型 愤怒 郁怒 旷场实验 糖水偏好实验 单胺类神经递质 

分 类 号：R228[医药卫生—中医基础理论]