乳腺癌癌前病变“毒瘀互结”模型大鼠生物表征变化及化瘀解毒法的调控  

Manifestation changes of the rat model with the poison and blood stasis syndrome of breast precancerous lesions and the regulation of eliminating stasis detoxification method

作　　者：邓卫芳[1] 裴晓华[2] 金华[1] 李倩[1] 梁晨[1] 赵乐[1] 

机构地区：[1]北京中医药大学,北京100029 [2]北京中医药大学第三附属医院,北京100029

出　　处：《中华中医药杂志》2013年第5期1508-1512,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：北京市自然科学基金资助项目(No.7102093)~~

摘　　要：目的:建立乳腺癌癌前病变"毒瘀互结"病证结合模型,对模型进行评价,并观察化瘀解毒法对该模型大鼠的调控作用。方法:SPF级SD雌性大鼠40只随机分为4组:空白组10只、对照组10只,给予二甲基苯蒽(DMBA)灌胃;模型组10只、西黄丸组10只,均给予DMBA灌胃+多重复应激10周。造模成功后,西黄丸组给予相应药物灌胃,1次/d,连续30d。于第1天、10周末、灌胃结束后分别观察并记录大鼠生物表征变化;腹腔取血观察血液流变学变化;Real-time PCR及Western blot法对乳腺组织血管内皮生长因子(VEGF)、趋化因子受体CXCR4基因及蛋白进行检测。结果:经过大鼠生物表征变化、血液流变学变化及病理变化、西黄丸药物反证证明大鼠乳腺癌癌前病变毒瘀互结模型造模成功。西黄丸组VEGF mRNA及蛋白、CXCR4 mRNA及蛋白表达水平均较模型组明显降低,差异有统计学意义(P<0.05),其中,VEGF mRNA表达水平降低更为明显(P<0.01)。结论:将疾病模型及中医病因致病模型结合制造乳腺癌癌前病变毒瘀互结模型符合临床患者发病过程,经评价造模成功;化瘀解毒法代表方西黄丸可逆转大鼠乳腺癌癌前病变,这种作用可能是通过抑制VEGF及CXCR4基因及蛋白表达,从而抑制血管生成及趋化因子受体表达而实现的。Objective： Establishing and evaluation of the rat model with the poison and blood stasis syndrome of breast precancerous lesions to observe the regulating effect of eliminating stasis detoxification. Methods： Forty SD rats of SPF degree were randomly divided into four groups： blank group （n=10）, control group （n=10）, model group （n=10） and Xihuang Pill group （n=10）. The poison and blood stasis syndrome of precancerous lesions of breast cancer model was induced in rats of the model group and Xihuang Pill group. The biological characterization changes of the rats were observed and recorded. After treatment for 30 days, the blood rbeology changes were observed. The expression of VEGF and CXCR4 were detected by real-time PCR and Western blot. Results： All the changes （biological characterization change, pathological changes and hemodynamic changes, Xihuang Pill drugs） of rats proved that the model was success. The expression of VEGF mRNA and protein and CXCR4 mRNA in the Xihuang Pill group were significantly lower than that in the control group; The protein expression of CXCR4 were significantly higher in Xihaung Pill group than in the control group （P〈0.05）. Conclusions： Combined with the disease model and TCM etiology pathogenesis manufacturing model of breast precancerous lesion poison and blood stasis syndrome, it conformed to the process of clinical disease which proved to be success. Xihuang Pill could reverse precancerous lesions of breast cancer which is possible achieved by inhibiting the VEGF and CXCR4 genes and proteins in rats with breast atypical hyperplasia so that inhibiting the expression of the chemotactic factor receptor.

关 键 词：西黄丸 乳腺癌癌前病变 毒瘀互结模型 病证结合造模法 模型评价 

分 类 号：R273[医药卫生—中西医结合]