一株新基因型猪圆环病毒Ⅱ型的鉴定  

Identification of a Novel Genotype in Porcine Circovirus Type Ⅱ

作　　者：谢俊岭[1] 陈晓平[1] 孟凡伟[1] 王保有[2] 周双海[1,2] 王志军[2] 

机构地区：[1]北京农学院动物科学技术学院,北京102206 [2]天津市宁河原种猪场,天津301504

出　　处：《中国农学通报》2013年第11期34-38,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"微血管内皮细胞在猪圆环病毒II型感染中的作用"(30600442);天津市宁河原种猪场科技计划"规模化猪场重要疫病防控关键技术研究"(2011)

摘　　要：为了解新近出现的猪圆环病毒Ⅱ型(PCV2)的基因变异及其基因型信息。用PCR技术从新近发生断奶后多系统消耗综合征的病猪组织中扩增和克隆出一株PCV2全基因组序列,并进行了遗传变异分析。结果显示:该PCV2毒株基因组全长为1767bp,与部分其他PCV2毒株的核苷酸同源性达94.0%以上,并显示出一定的地域、时间相关性。病毒ORF2基因系统发育进化分析显示:该PCV2毒株与已有PCV2基因型的遗传距离均大于0.035,因而被划入到一个新的PCV2基因型,暂命名为PCV2d,属于在中国新近出现的一种PCV2基因型。研究结果表明,克隆鉴定了一株新基因型PCV2。The aim of the present study was to investigate the genetic variation and genotype information of a newly emerging Porcine Circovirus Type 2 （PCV2） strain. The complete genome sequence of one PCV2 strain was amplified and cloned from tissues of pig suffered newly from postweaning multisystemic wasting syndrome by PCR assay. Then, the genetic variation of several PCV2 strains was analyzed. The results showed that the complete genome of this cloned PCV2 strain was 1767 base pair in size, and shared more than 94.0% nucleotide identity with several other PCV2 strains. Moreover, there were some correlation of geography and time among the selected PCV2 strains. The phylogenetic analysis results of the ORF2 genes of these PCV2 strains revealed that the genetic distance between the cloned PCV2 strain and the existed PCV2 genotypes was all more than 0.035, so it was assorted into a novel genotype named PCV2d temporarily, which was a newly emerging PCV2 genotype in China. The research results indicated that one strain of new genotype PCV2 was cloned and identified.

关 键 词：猪圆环病毒II型 基因变异 进化分析 基因型 

分 类 号：S852.659.2[农业科学—基础兽医学]