人参皂苷Rbl对阿霉素心力衰竭大鼠心肌细胞核因子-κB的影响  

Effects of Ginsenosides-Rbl on nuclear factor kappa B in rats with chronic heart failure induced by adriamycin

作　　者：孔宏亮[1] 苗志林[1] 郭翠艳[1] 孟锦[1] 

机构地区：[1]辽宁省人民医院心脏中心,辽宁沈阳110016

出　　处：《中国药理学通报》2013年第4期573-576,共4页Chinese Pharmacological Bulletin

基　　金：辽宁省自然科学基金资助项目(No 201102107)

摘　　要：目的探讨人参皂苷Rbl(Gs-Rb1)改善阿霉素(Adr)慢性心力衰竭(CHF)效应是否与抑制核因子-κB(NF-κB)有关。方法 Adr诱导CHF大鼠模型被随机分为Adr组(n=15)和Gs-Rbl组(70 mg.kg-1.d-1,n=17),另随机选取同龄健康大鼠作为对照(n=10);同时培养乳鼠心肌细胞并随机分为对照组、Adr组(1μmol.L-1)和Gs-Rbl组(1μmol.L-1Adr+200μmol.L-1 Gs-Rbl)。干预完毕后,检测心脏超声、NF-κB、IκBα、p-IκBα和NF-κB DNA结合活性。结果 (1)Gs-Rb1组LVEF明显改善(P=0.003);(2)在NF-κB p50和NF-κB p65方面,体内或体外的Gs-Rbl组均明显低于Adr组(P<0.001);(3)体内、体外的Gs-Rbl组NF-κBDNA结合活性明显低于Adr组(P<0.01);(4)体内、体外的Adr组IκBα蛋白明显高于对照组,而Gs-Rbl组IκBα最高(P<0.01);Gs-Rbl组p-IκBα和p-IκBα/IκBα比值明显低于Adr组(P<0.01)。结论 Gs-Rb1改善CHF效应与其抑制NF-κB活性有关。Aim Based on the adriamycin（Adr）-induced chronic heart failure（CHF）,the present study elucidates the effect of Ginsenosides-Rbl（Gs-Rb1） on nuclear factor kappa B（NF-κB）,in vivo and in vitro.Methods CHF rats were randomly divided into Adr group（n=15） and Gs-Rb1 group（70 mg·kg-1·d-1,n=17）,and the age-matched rats as control（n=15）;in addition,cardiomyocytes were randomly divided into control group,Adr group（1 μmol·L-1） and Gs-Rb1 group（1 μmol·L-1 Adr＋200 μmol·L-1 Gs-Rb1）,too.After the above intervention was performed,the left ventricular ejection fraction（LVEF） was analyzed by echocardiography,in vivo,the expression of NF-κB,IκBα,p-IκBα and NF-κB DNA binding activity were assayed.Results（1） LVEF was significantly improved in Gs-Rb1 group than in Adr group（P=0.003）.（2） Both NF-κB p50 and NF-κB p65 were significantly down-regulated in Gs-Rb1 group than in Adr group（P＆lt;0.01）,in vivo and ex vivo.（3） NF-κB DNA binding activity was significantly down-regulated in Gs-Rb1 group than in Adr group（P＆lt;0.01）,in vivo and ex vivo.（4） IκBα protein was significantly up-regulated by Adr,especially by Gs-Rb1,compared with control（P＆lt;0.01）,in vivo and in vitro;both p-IκBα protein and p-IκBα/IκBα ratio were significantly lower in Gs-Rb1 group than in Adr group,in vivo and in vitro.Conclusion The effects of Gs-Rb1 on the improvement of CHF were partly performed by means of inhibiting NF-κB at least.

关 键 词：人参皂苷RBL 阿霉素 慢性心力衰竭 核因子-ΚB 核因子-κB抑制蛋白 核因子-κBDNA结合活性 

分 类 号：R-332[医药卫生] R284.1