肿瘤细胞中Ro60的表达及其对肿瘤细胞辐射敏感性的影响  

Ro60 overexpression contributes to proliferation and sensitivity of tumor cells to γ-irradiation

作　　者：楼敏铭[1,2] 张兴[2,3] 田宝磊[2] 刘继来[2] 刘斌[2] 张令强[1,2] 郑晓飞[2] 宋宜[2] 孙志贤[2] 

机构地区：[1]安徽医科大学研究生院,合肥230039 [2]军事医学科学院放射与辐射医学研究所,北京100850 [3]第四军医大学基础部,西安710032

出　　处：《军事医学》2013年第4期241-245,共5页Military Medical Sciences

基　　金：国家自然科学基金资助项目(30900167)

摘　　要：目的检测肿瘤细胞中RNA结合蛋白Ro60的表达定位,研究Ro60在肿瘤增殖和辐射敏感性中的作用。方法构建带绿色荧光素酶标签的Ro60真核表达载体,转染肿瘤细胞,观察电离辐射引发的DNA损伤反应中Ro60的表达定位规律;CCK8和锥虫蓝染色实验检测Ro60蛋白对肿瘤细胞增殖和辐射敏感性的影响。结果多种肿瘤细胞中均有较高水平的Ro60蛋白表达。γ射线诱导的DNA损伤反应中Ro60发生显著的表达上调和亚细胞定位改变。过表达Ro60的肿瘤细胞增殖延缓,细胞死亡明显增多,辐射引发的细胞增殖抑制和死亡显著增强。结论辐射引发肿瘤细胞中Ro60表达和定位改变,调控肿瘤细胞增殖和凋亡,提高肿瘤细胞的辐射敏感性。Objective To investigate the expression and subcellular localization of RNA-binding protein Ro60 in neoplasms before and after γ-ray irradiation,and the function of Ro60 in tumor cell proliferation and radio-sensitivity.Methods The eukaryotic expression plasmid of mGFP-Ro60 was constructed and transfected into HCT116 and MCF7 tumor cells.The cellular localization of Ro60 was examined before and after irradiation.Cell proliferation and radio-sensitivity were detected by CCK8 and trypan blue assay.Results The result of immunoblotting showed that tumor cells expressed Ro60 protein.The irradiation increased Ro60 expression and induced significantl nuclear aggregation of Ro60.The cell proliferation before and after irradiation was drastically reduced while cell death increased in Ro60 overexpressed tumor cells.Conclusion γ-ray irradiation alters Ro60 expression and localization,and Ro60 plays an important role in tumor cell proliferation and radio-sensitivity.

关 键 词：Ro60 肿瘤 细胞增殖 辐射敏感性 

分 类 号：R730.55[医药卫生—肿瘤]