抗人前列腺干细胞抗原单克隆抗体的制备及筛选  

Preparation and selection of monoclonal antibodies against human prostate stem cell antigen

作　　者：樊红艳[1] 刘树玲[1] 张晓鹏[1] 易绍琼[1] 房婷[1] 董磊[1] 于长明[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京10007

出　　处：《军事医学》2013年第4期259-262,共4页Military Medical Sciences

基　　金：国家自然科学基金资助项目(81172980)

摘　　要：目的获得能够结合细胞表面前列腺干细胞抗原(prostate stem cell antigen,PSCA)的抗人PSCA单克隆抗体。方法以重组人PSCA蛋白为免疫原,通过杂交瘤技术制备抗人PSCA单克隆抗体,并进行ELISA、Western印迹、流式细胞术等实验筛选。结果获得6株抗人PSCA单克隆抗体,经过筛选最终得到3株能够结合细胞表面PSCA的单克隆抗体。结论获得具有结合细胞表面PSCA活性的单克隆抗体,为进一步研究和应用奠定了基础。Objective To prepare and select monoclonal antibodies（mAb） against human prostate stem cell antigens（PSCA） in cells.Methods BALB/c mice were immunized with recombinant human PSCA.Anti-PSCA mAbs were obtained by hybridoma technique,tested by ELISA,Western blotting and flow cytometry.Results Six strains of anti-PSCA mAbs were acquired,three of which proved to be able to bind PSCA in cells after all tests.Conclusion Acquisition of anti-PSCA mAbs able to bind PSCA on the cell surface will facilitate further studies and applications.

关 键 词：前列腺肿瘤 肿瘤干细胞 抗体 单克隆 

分 类 号：R737.25[医药卫生—肿瘤]