西番莲果皮总黄酮提取工艺及抑制亚硝化反应  

Microwave-assisted Extraction and Nitrosation Inhibition Activity of Total Flavonoids from Passiflora edulis Rind

作　　者：冯纪南[1] 黄海英[2] 赵丽萍[1] 章爱华[3] 邓斌[1] 

机构地区：[1]湘南学院化学与生命科学系,湖南省郴州市苏仙北路8号423000 [2]湘南学院教务处,湖南省郴州市苏仙北路8号423000 [3]吉首大学化学化工学院,湖南吉首市人民南路120号416000

出　　处：《光谱实验室》2013年第3期1179-1184,共6页Chinese Journal of Spectroscopy Laboratory

基　　金：湖南省自然科学基金项目(12JJ3019);湖南省科技厅计划项目(2010GK3123;湖南省教育厅重点科研基金项目(10A113);郴州市科技计划项目(郴财教指[2011]53号)

摘　　要：运用正交试验设计探讨了西番莲果皮总黄酮的微波辅助提取工艺条件,并采用分光光度法研究了提取液对合成亚硝胺的抑制能力和清除亚硝酸盐的能力。结果表明,西番莲果皮总黄酮提取的最佳工艺参数:乙醇体积分数60%、料液比1∶40(g/mL)、微波辐射时间15min、微波炉功率400W,在此条件下总黄酮的提取率为57.03mg/g。对西番莲果皮总黄酮提取液的抑制亚硝化反应研究表明,提取液对亚硝胺合成的最大阻断率为80.8%,对亚硝酸钠的最大清除率为73%。The optimal the extract technology of total flavonoids from Passiflora edulis Rind with microwave-assisted method were studied by orthogonal test and their abilities to inhibit nitrosamines and scavenge nitrite were tested spectrophotometrically. The best extraction condition was alcohol concentration 60 %, material-liquid rate 1 ： 40 （g/mL）, microwave irradiation time 15 rain, microwave output power 400 W. Under this condition, the extracting rate of flavonoids was 57.03mg/g. The maximum capabilities of inhibiting the production of nitrosodinethylamine and scavenging sodium nitrite were 80.8 % and 73 %,respectively.

关 键 词：西番莲果皮 总黄酮 微波辅助提取 亚硝酸盐 阻断 清除 

分 类 号：O623.54[理学—有机化学] O657.32[理学—化学]