超高效液相色谱法快速分离测定糖精钠、芝麻酚、酸性橙Ⅱ和碱性嫩黄O  

Simultaneous Determination of Sodium Saccharin,Sesamol,Acid Orange Ⅱ,Auramine O by Ultra High Performance Liquid Chromatography

作　　者：李晶[1,2] 李雪梅[1] 徐济仓[1] 缪明明[1] 陈建华[1] 杨叶昆[1] 

机构地区：[1]云南烟草科学研究院 [2]工业控制技术国家重点实验室浙江大学智能系统与控制研究所,浙江省杭州市浙大路38号310058

出　　处：《光谱实验室》2013年第3期1311-1316,共6页Chinese Journal of Spectroscopy Laboratory

基　　金：云南中烟公司科研资助项目(No.2012JC05)

摘　　要：建立了超高效液相色谱-二极管阵列检测器(UPLC-PDA)同时分离测定香精香料中糖精钠,芝麻酚,酸性橙Ⅱ,碱性嫩黄O的方法。样品经过10%的甲醇水溶液(含1%氨水)提取后,以Waters BEHC18(1.7μm,2.1mm×50mm)为色谱柱,5mmol/L乙酸铵(含0.1%乙酸)和乙腈(ACN)为流动相,200—500nm波长范围内进行扫描检测。结果表明,在5min以内4种目标物质得到了较好的分离,在0.5—20mg/L范围内具有较好的线性(r>0.9950),检出限为0.08—0.61mg/L,3种浓度水平的加标回收率保持在85.3%—110.3%之间,相对标准偏差在6%以内。该方法操作简单,灵敏度高,可以满足香精香料中4种物质的同时检测。An ultra high performance liquid chromatography method coupled with Photo DiodeArray Detector （PDA） as detector was established for the separation and determination of 4 substances （sodium saccharin,sesamol,acid orange Ⅱ,auramine O） in flavor and fragrant samples. After extracted by 10% methanol/water solution （containing 1% ammonia V/V）, the target compounds were separated on a Waters BEH C18 column （1. 7lμm, 2. 1ram X 50mm） using HPLC acetonitrile and 5 mmol/L ammonium acetate aqueous solution （with 0. 1%（V/V）） acetic acid） as mobile phases,and detected by a diode array detector scanned from 200 nm to 500 nm. It＇s exhibited that there was a good linear relationship （r〉0. 9950） ranged from 0. 5 to 20mg/L and the limits of detection was in range of 0. 08-0. 61mg/L. The average recoveries at three levels were in the range of 85.3%-110. 3% with the relative standard deviations （RSD）less than 6%. This simple method can be used to determine 4 substances in flavor and fragrant samples simultaneously.

关 键 词：超高效液相色谱 糖精钠 芝麻酚 酸性橙Ⅱ 碱性嫩黄O 分离 

分 类 号：O657.72[理学—分析化学]