特异性核基质结合区结合蛋白-1在甲状腺癌中的表达及促进甲状腺癌侵袭能力的研究  

Expression of special AT-rich sequence binding protein-1 in thyroid cancer and effect of special AT-rich sequence binding protein-1 on the invasion of thyroid cancer cells

作　　者：龚龙[1] 孙圣荣[1] 易春华[2] 郑建伟[3] 薛新波[3] 

机构地区：[1]武汉大学人民医院乳腺甲状腺外科,430060 [2]华中科技大学同济医学院附属荆州医院甲状腺外科 [3]华中科技大学同济医学院附属同济医院胆胰外科

出　　处：《中华实验外科杂志》2013年第5期937-939,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察特异性核基质结合区结合蛋白-1（SATB1）在人甲状腺癌组织中的表达及其对甲状腺癌细胞侵袭能力的影响。方法应用免疫组织化学链菌素抗生物素蛋白-过氧化物酶（SP）方法检测85例甲状腺癌手术标本癌旁及癌组织中SATB1的表达水平，采用χ^2检验分析其与临床病理参数的关系。在甲状腺癌细胞SW-579中转染SATB1基因，检测过表达SATB1对甲状腺癌细胞增殖及侵袭能力的改变。结果SATB1主要在细胞核中表达，在85例甲状腺癌组织中，有58例高表达（高表达率为68．2％）；SATB1的高表达与性别、年龄、肿瘤大小以及病灶多少无明显相关（P〉0．05），而与淋巴转移和病理分期有关（P〈0．05）。细胞侵袭实验结果显示，转染空载体组与转染SATB1重组体质粒组透膜细胞数分别为（220±10）个和（416±15）个，过表达SATB1显著促进甲状腺癌细胞SW-579的侵袭能力（P〈0．05）。结论甲状腺癌组织中高表达的SATB1与肿瘤侵袭转移有关。Objective To observe special AT-rich sequence binding protein-1 （SATB1） expression in thyroid cancer and the effect of SATB1 on invasion of thyroid cancer cells. Methods The expression of SATB1 in 85 cases of thyroid cancer was detected by using immunohistochemistry. The correlation between SATB1 and clinicopathological features was analyzed by Chi-Squared test. The effect of SATB1 on invasion of SW579 cells was analyzed by using invasion assay. Results The positive expression of SATB1 in thyroid cancer was 68.2% （58/85）. SATB1 was mainly expressed in nucleus of thyroid cancer cells. SATB1 expression was associated with lymph node metastasis and pathological stage （ P 〈 0. 05 ）, but not associated with sex, age, tumor sizes and number of tumor nodules （ P 〉 0. 05）. Invasion assay showed that the migrating number of empty vector transfected group and pEGFP1-SATBI transfected group was respectively （220 ± 10） and （416 ±15） （P 〈 0. 05）. Conclusion The up-regulation of SATB1 in thyroid cancer has a close relationship to the invasion of thyroid cancer ceils.

关 键 词：甲状腺肿瘤 核基质蛋白 特异性核基质结合区结合蛋白-1 肿瘤转移 

分 类 号：R736.1[医药卫生—肿瘤]