滑膜间充质干细胞在五种不同趋化因素作用下迁移的研究  

The experimental study on migration of synovium-derived mesenchymal stem cells by five chemo tactic factors

作　　者：潘建锋[1] 郭常安[1] 沈剑锋[2] 刘嘉[1] 阎作勤[1] 

机构地区：[1]复旦大学附属中山医院骨科,上海200032 [2]复旦大学材料科学系

出　　处：《中华实验外科杂志》2013年第5期1013-1015,共3页Chinese Journal of Experimental Surgery

基　　金：基金项目：国家自然科学基金资助项目（81071468、81171671）

摘　　要：目的观察血清、转化生长因子（TGF）-β3、血小板衍生生长闪子（PDGF）-AB、骨形态发生蛋白（BMP）-2、成纤维细胞生长因子（FGF）对兔滑膜间充质干细胞（SMSCs）迁移的影响。方法选取含浓度0．1％、0．5％、1．0％、2．0％、5．0％、10．0％、20．0％血清以及含50μg/LTGF-β3、PDGF—AB、BMP-2、FGF的低糖DMEM作用于兔SMSCs，Transwell小室检测其迁移能力。结果 血清、TGF-β3、PDGF—AB、BMP-2、FGF均可趋化兔SMSCs迁移，血清浓度为5．0％时趋化作用较其他浓度组明显，每×100倍视野下迁移细胞数为（169．00±21．74）个，4种因子中PDGF—AB趋化作用较TGF—β3、BMP-2、FGF明显（219．83±11．29比84．50±10．33、146．67±17．91、99．37±12．27）。结论兔SMSCs可在TGF-β3、PDGF—AB、BMP-2、FGF作用下迁移，其中PDGF—AB趋化最明显，可用于促进SMSCs迁移归巢，进行软骨原位冉生的修复研究。Objective To explore the chemotactic migration effects of serum, transforming growth factor （TGF）-β3, platelet derived growth factor （PDGF）-AB, bone morphogenetic protein （BMP）-2, and fibroblast growth factor （FGF） on rabbit synovium-derived mesenchymal stem cells （SMSCs）. Methods The SMSCs were obtained with passage isolation, and then stimulated by low-glucose DEME containing TGF-β3, PDGF-AB, BMP-2, FGF with 50 p^g/L concentration as well as 0. 1% , 0. 5% , 1.0% , 2.0% , 5.0%, 10. 0%, 20. 0% serum. The migration of SMSCs under five different ehemotaetic factors was observed in Transwell chamber assays. Results TGFq33, PDGF-AB, BMP-2, FGF and serum consistently enhanced the migration activity of rabbit SMSCs. Compared with other concentration, 5.0% is the appropriate concentration for serum to induce the migration of rabbit SMSCs. The average numbers of migratory cells （SMSCs） at × 100 magnification was 169. 00 ±21.74 in lower chamber under the 5.0% serum within the Transwell assay. Among the four growth factors, PDGF-AB showed more potent chemotactic effect than otherthree （219.83 ±11.29 vs. 84.50±10.33, 146.67±17.91, 99.37 ±12.27）. Conclusion The migration of SMSCs from rabbit could be enhanced by TGF-β, PDGF-AB, BMP-2 and FGF. PDGF-AB is the most potent chemotactic factor among the four growth factors, and may he useful to promote cell homing and cartilage regeneration in situ during cartilage repair.

关 键 词：滑膜间充质干细胞 细胞因子 趋化 迁移 

分 类 号：R77[医药卫生—眼科]