急性淋巴细胞白血病患儿骨髓基质细胞的粘附行为  

Adhesion of bone marrow stromal cell in children with acute lymphoblastic leukemia

作　　者：黄耘[1] 李树浓[2] 李晓瑜[1] 林穗珍[1] 罗学群[1] 覃肇源[1] 

机构地区：[1]中山医科大学第一附属医院儿科,广东广州510080 [2]中山医科大学病理生理教研室,广东广州510089

出　　处：《中国病理生理杂志》2000年第8期713-717,共5页Chinese Journal of Pathophysiology

摘　　要：目的和方法 :用MTT方法检测了急性淋巴细胞白血病 (ALL)患儿骨髓基质细胞 (BMSC)对正常骨髓造血细胞和淋巴瘤Raji细胞的粘附行为 ,用流式细胞仪检测了BMSC表面的粘附分子ICAM - 1和VCAM -1的表达。结果 :急性期组ALLBMSC对骨髓造血细胞的粘附能力明显下降。长期缓解组ALLBMSC对肿瘤细胞的粘附能力明显增高。急性期组ALLBMSC表面ICAM - 1表达明显降低。结论 :ALL患儿BMSC对骨髓造血细胞和肿瘤细胞的粘附行为的异常 。AIM and METHOD: The adhesion behavior of bone marrow stromal cells(BMSC) in children with acute lymphoblastic leukemia(ALL) to bone marrow mononuclear cells(BMMC) and Raji cells were investigated by MTT method. The expression of adhesion molecules ICAM-1 and VCAM-1 were detected by flow cytometry. RESULTS: The adhesion ability of BMSC in acute period of ALL to BMMC was lower than that of control group. The adhesion ability of BMSC in long term remission period of ALL to Raji cells higher than that of control group. The expression of ICAM-1 on the surface of BMSC in acute period of ALL is much lower than that of control group. CONCLUSIONS: The adhesion of BMSC to BMMC or tumor cells in children with ALL was abnormal. The abnormal adhesion behavior might be partially due to the changed expression of adhesion molecules on BMSC.

关 键 词：急性淋巴细胞白血病 细胞粘附分子 骨髓基质细胞 

分 类 号：R733.710.2[医药卫生—肿瘤]