δ阿片受体激动剂DADLE对大鼠急性全脑缺血再灌注肺水转运的作用  

Effect of the δ-opioid Receptor Agonist DADLE on lung water transport after Global Cerebral Ischemia and Reperfusion in Rats

作　　者：黄伟青[1] 武钊[1] 文锐玲[1] 刘佩仪[1] 欧阳斌[1] 郭伟[1] 梁庆[1] 

机构地区：[1]广州医学院第一附属医院,510120

出　　处：《中国医疗前沿》2013年第7期33-34,4,共3页China Healthcare Innovation

基　　金：2011年广州市大学生科技创新实践项目(编号:1057011004);广州市医药卫生科技项目(编号:201102A213152)

摘　　要：目的通过二血管阻断加低血压法建立大鼠全脑缺血再灌注模型,观察δ阿片受体激动剂DADLE对大鼠肺水转运的作用,探讨其对全脑缺血再灌注肺损伤(ALI)的保护作用。方法健康雌性SD大鼠30只随机分为假手术组(Sham)、模型组(I/R)、DADLE处理组各10只。假手术组(Sham):只暴露血管而不夹闭;缺血再灌注组(I/R):采用改良的二血管阻断加低血压法建立全脑缺血再灌注模型,缺血15min后给予再灌注120min;DADLE处理组:于再灌注前经由左侧颈静注射DADLE5mg/kg,再灌注120min后,取右肺组织,常规HE染色观察其形态学改变,左肺叶组织湿质量/干质量(W/D)值。并与假手术组(Sham)、模型组(I/R)进行对照分析。结果成模后的肺脏表现为肺泡间隔增宽,毛细血管扩张充血,肺胞腔内及血管周围中性粒细胞浸润,气管壁部分上皮脱落,肺胞腔及气管腔均有浆液渗出。DADLE处理组肺脏充血减轻,中性粒细胞浸润有所减少。结论大鼠急性全脑缺血再灌注模型对肺有不同程度的损伤,DADLE可减少肺组织液体过量分泌,对ALI具有一定的保护作用。Objective To occlusion combined with hypotension to establish the rat model of cerebral ischemia reperfusion by two vessels, observation of δ opioid receptor agonist DADLE on the transport of water in the lung in rats, to explore its on cerebral isehemia reperfusion injury（ALI） and the protective effect of. Methods 30 healthy SD rats were randomly divided into sham operation group（Sham）, model group（I/R）, DADLE treatment group. Sham operation group（Sham, n-10）： expose only blood vessels and no closed, ischemia reperfusion group（I/R, 1）=10）; using improved two vessel occlusion of cerebral ischemia reperfusion model combined with hypotension, 15rain after ischemia reperfusion was given 120min; DADLE treatment group（n=10） on reperfusion first through the left jugular venous injection of DADLESmg/kg, 120min after reperfusion, and the right lung tissue, morphological changes were observed by routine HE staining, left lung tissue wet weight/dry weight（W/D） value. Compared with the sham operation group（Sham）, model group（I/R） were analyzed. Results After injection of the lung showed alveolar septum, capillary dilatation and congestion, alveolar cavity and perivascular infiltration of neutrophils, tracheal wall portion of epithelial cell, lung and tracheal cavity are serous fluid exudation. DADLE treatment group reduced lung congestion, neutrophil infiltration decreased. Conclusion Acute cerebral ischemia reperfusion rat models with different degrees of damage to the lungs, DADLE can reduce lung fluid excess secretion, has a protective effect on ALI.

关 键 词：DADLE 全脑缺血再灌注 肺损伤 肺水转运 

分 类 号：R364.1[医药卫生—病理学]