镝(Dy)-L-苯丙氨酸-5-氟尿嘧啶三元配合物的合成及与牛血清白蛋白相互作用的研究  

Synthesis and interaction of Dy-L-phe-5-fu ternary complex with bovine serum albumin at molecular level

作　　者：李晓慧[1,2] 王卫东[1,2] 金传明[1,2] 陆江林[1] 

机构地区：[1]湖北师范学院污染物分析与资源化技术湖北省重点实验室,湖北黄石435002 [2]湖北师范学院化学与环境工程学院,湖北黄石435002

出　　处：《化学试剂》2013年第5期405-409,431,共6页Chemical Reagents

基　　金：国家自然科学基金资助项目(21171053);湖北省教育厅重点科研资助项目(D20082202);湖北师范学院研究生创新研究资助项目(201203)

摘　　要：利用紫外-可见吸收光谱和荧光光谱法,在Tris-HCl缓冲溶液中考查了不同温度下镝(Dy)-L-苯丙氨酸-5-氟尿嘧啶三元配合物与牛血清白蛋白(BSA)之间的相互作用。实验表明,配合物对BSA的内源荧光猝灭为静态猝灭过程,并测定该反应在不同温度下的结合常数KA,且配合物与BSA以物质的量比1∶1结合。根据计算出的热力学参数表明,配合物和BSA之间的作用力主要是静电作用。用同步荧光技术考察了配合物对BSA构象的影响,结合位点接近于酪氨酸残基。The interaction between Dy-L-phe-5-fu ternary complex and bovine serum albumin （BSA） was investigated via ultraviolet-visible absorption spectra and fluorescence in tris-HC1 buffer solutions at different temperatures. The results showed that static quenching was involved in adding complexin BSA solution. The complex reacted at a molar ratio of 1 ： 1 and the binding constants （Ks ） were determined. The interaction between complex and BSA was driven mainly by electrostatic interactions according to thermodynamic parame- ters. Respectively, the effect of ternary complexes on the conformation of BSA was obtained using synchronous fluores- cence spectroscopy. The binding site is close to the tyrosine residue.

关 键 词：三元稀土配合物 合成 牛血清白蛋白 相互作用 

分 类 号：O641.3[理学—物理化学]