一株耐高温抑菌黏质沙雷氏菌的鉴定及其红色素的初步分离  

Identification of Thermo-stability Serratia marcescens Strain Inhibiting Bacterial and Preliminary Isolation of Red Pigment

作　　者：赵银娟[1] 薛斌[2] 李桂娥[1] 吴小扁[1] 

机构地区：[1]南京林业大学森林资源与环境学院江苏省有害生物入侵与控制重点实验室,江苏南京210037 [2]南京大学医学院,江苏南京210037

出　　处：《安徽农业科学》2013年第7期2829-2831,共3页Journal of Anhui Agricultural Sciences

基　　金：国家自然科学青年基金(31100448);博士点基金(21132-04120004);江苏高校优势学科建设工程资助项目

摘　　要：[目的]为了了解黏质沙雷氏菌的抑菌机理及温度对其产生色素的影响。[方法]从土壤中分离纯化得到1株产红色素细菌,对该菌进行生理生化和16S rDNA鉴定,同时对该菌产生的红色素进行了初步的探讨。[结果]该菌为黏质沙雷氏菌(Serratia marcescens),在28℃条件下培养红色素产量最高,37℃下仍能产生色素,说明该菌株是耐高温红色素产生菌。紫外全波长扫描分析和薄板层析结果表明,其红色色素有可能是灵菌红素。[结论]该菌对霉状杆菌和镰刀菌等病原菌具有一定的抑制作用。[Objective]The research aimed to study the antibacterial mechanism of Serratia marcescens and the effects of the temperature on the production of the red pigment.[Method] A bacterial strain producing red pigment was isolated from soil,and its morphology,biochemical,physiological characteristics and 16S rDNA sequence were investigated.[Result] The strain was identified as Serratia marcescens.The red pigment was extracted by ethyl alcohol.The maximum production was obtained at 28 ℃,still,a little pigment were synthesized at 37 ℃.This result indicated that the strain was temperature-stability.The results of ultraviolet full wavelength scanning analysis and sheet chromatography showed that the red pigment might be prodigiosin.[Conclusion] Serratia marcescens could inhibit bacterial such as fusarium and bacillus cereus mycoides.

关 键 词：红色素 黏质沙雷氏菌 鉴定 

分 类 号：S182[农业科学—农业基础科学] Q93[生物学—微生物学]