CD_(44)v_6修饰DC融合瘤苗体外抗结肠癌作用的研究  

The Anticolon-cancer Research of Fusion Hybrid of Dendritic Cells Expressing CD44v6 in Vitro

作　　者：赵光艳 胡显芳 李彦[2] 

机构地区：[1]重庆市涪陵区人民医院内一科,重庆408000 [2]第三军医大学西南医院消化科,重庆400038

出　　处：《中国医药指南》2013年第7期408-409,共2页Guide of China Medicine

摘　　要：目的小鼠提取的树突状细胞(DC)和小鼠结肠癌细胞CT26.WT共培养、融合,CD44v6基因来修饰融合细胞,研究CD44v6修饰DC融合瘤苗体外抗结肠癌的作用机制,为肿瘤预防及免疫治疗提供实验依据。方法①在聚乙二醇的作用下,DC细胞和CT26.WT细胞共培养、融合,利用HAT/HT筛选系统筛选出纯净DC融合细胞。在脂质体的作用下,将pBud-CD44v6转染到DC融合细胞中表达;②体外实验观察DC融合瘤苗对小鼠结肠癌细胞的杀灭作用。结果构建了基因CD44v6修饰的DC/CT26.WT肿瘤融合疫苗;实验组细胞的淋巴细胞毒(CTL)活性明显高于对照组。结论 CD44v6修饰的DC融合瘤苗可有效诱导T淋巴细胞产生强大抗结肠癌作用。Objective To survey the antitumor mechanism and effect of fusion hybrid cells of DCs and colon cancer cell CT26.WT co-cultivating and expressing CD44v6 gene, DCs and colon cancer cell CT26.WT derived from mice were blended, and modified with CD44v6 gene, it will provide the experimental foundation on the prevention and immunotherapy for tumor patients. Methods ①CT26.WT cells and DCs were blended by polyethylene glycal, and the fused cells were screened by HAT/HT system. The recombinant pBud-CD44v6 vector was transfected into the fused cells by lipofectamine, and to express; ②The anticancer effect and cytotoxicty of fused-cell vaccine were studied in vitro. Result DC/CT26.WT fused cell vaccine modified with CD44v6 geng was constructed; the lymphocyte cytotoxicity T （CTL） of immune group was larger activity than that of the anainst group .Conclusion The fused cells of DC expressing CD44v6 geng can induce strong anticolon-cancer activity ofT lymphocyte.

关 键 词：CD44V6基因 树突状细胞 细胞融合 结肠癌 

分 类 号：R735.35[医药卫生—肿瘤]